接触电子烟会破坏呼吸道上皮质屏障的功能,并加剧病毒感染
Andjela Raduka1, Nannan Gao1, Robert L Chatburn2
1Department of Inflammation and Immunity, Lerner Research Institute, Cleveland Clinic Foundation, Cleveland, Ohio, United States.
American journal of physiology. Lung cellular and molecular physiology
|September 12, 2023
概括
电子烟 (e-cig) 的使用会损害呼吸道上皮质屏障,增加对呼吸道同胞病毒 (RSV) 感染的易感性. 电子烟的成分,包括尼古丁和风味,破坏肺部防御,并加剧病毒损伤.
科学领域:
- 肺部医学 肺部医学
- 毒理学 毒理学 毒理学
- 细胞生物学 细胞生物学
背景情况:
- 电子烟 (e-cig) 的使用是一个流行病,特别是在青少年中,构成公共卫生风险.
- 电子烟对呼吸道上皮质屏障的影响,对抗病原体的防御至关重要,仍然不清楚.
- 呼吸道同胞体病毒 (RSV) 感染会破坏呼吸道上皮质屏障的功能,导致住院病例的增加.
研究的目的:
- 研究电子烟组件如何影响呼吸道上皮质屏障的完整性.
- 为了确定电子烟暴露是否会加剧RSV诱导的呼吸道屏障功能障碍.
- 评估尼古丁和风味气溶对呼吸道上皮细胞的影响.
主要方法:
- 人类支气管上皮细胞 (16HBE和NHBE) 暴露在电子烟尼古丁提取物和气溶中.
- 分析了跨体电阻 (TEER) 和顶峰结合复合体 (AJC) 结构.
- 西方黑斑被用来评估AJC蛋白质水平.
- 细胞在暴露于尼古丁气溶后被RSV感染.
主要成果:
- 在非细胞毒性度下,电子烟尼古丁暴露显著降低了TEER,并破坏了AJC结构.
- 气溶中的肉和薄荷风味加剧了尼古丁诱导的屏障功能障碍.
- 前期暴露于尼古丁喷雾剂增加了RSV感染,并加剧了RSV诱导的呼吸道屏障破坏.
结论:
- 包括尼古丁和风味成分在内的电子烟暴露会破坏呼吸道上皮质屏障.
- 电子烟会加剧RSV引起的呼吸道损伤,并增加对病毒感染的易感性.
- 这些发现突出了电子烟对呼吸系统的不利影响,并为公共卫生战略提供了信息.
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