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缺少specc1l导致异常的卵细胞半离合,并减少了小鼠胚胎细胞的发育

Seulah Lee^1,2, Inchul Choi¹

¹Division of Animal and Dairy Sciences, College of Agriculture and Life Sciences, Chungnam National University, Republic of Korea.

Reproduction (Cambridge, England)

|September 12, 2023

概括

蛋白质SPECC1L对于小鼠卵细胞的适当成熟和早期胚胎发育至关重要. 它的淘汰会导致轴组装和染色体对齐的缺陷,影响发育能力.

科学领域:

生殖生物学生殖生物学
细胞生物学细胞生物学
发展生物学发展生物学

背景情况:

卵细胞成熟对于成功的早期胚胎发育至关重要.
异常的介质成熟会对植入前胚胎的发育能力产生负面影响.

研究的目的:

调查SPECC1L (具有卡尔波宁同质性和卷状卷状域1的精子抗原) 在小鼠卵细胞中介成熟中的作用.
确定SPECC1L对植入前发育的影响.

主要方法:

在卵细胞成熟期间分析Spec1l转录水平.
对SPECC1L蛋白质的免疫检测.
在卵细胞中 Specc1l 表达的抑制.
评估形态,染色体对齐,极体挤出和胚胎细胞形成率.

主要成果:

Specc1l转录水平从胚芽囊到第II阶段保持稳定,而母体转录在受精后降低.
SPECC1L局限于细胞质和生殖囊泡,而不是核状体.
在SPECC1L中断导致了异常的形态,染色体不对齐,极体挤出减少,胚胎细胞形成减少.
在第II阶段,SPECC1L没有与染色质共定位.

结论:

在小鼠卵细胞化过程中,SPECC1L对于正确的双极旋组装至关重要.
在第一个极体的挤出中,SPECC1L起着至关重要的作用.
SPECC1L对于小鼠卵细胞和早期胚胎的发育能力至关重要.

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