调节细胞分化,以促进Aureobasidium pullulans NG中普卢兰的合成
Nan Zeng1, Ning Zhang2, Dandan Wang3
1College of Land and Environment, Shenyang Agricultural University, Shenyang, 110866, People's Republic of China.
Applied microbiology and biotechnology
|September 12, 2023
概括
酸 (CA) 调节Aureobasidium pullulans NG中的细胞分化,促进胀的细胞生长,并显著增加了pullulan的产生. 这一发现为提高生物聚合物产量提供了一个有希望的策略.
科学领域:
- 微生物学 微生物学
- 生物技术是生物技术.
- 生物化学 生物化学
背景情况:
- 普鲁兰是一种有价值的生物聚合物,由Aureobasidium物种生产.
- 普鲁兰的产量受到Aureobasidium细胞分化的影响,Aureobasidium细胞分化对环境变化敏感.
- 了解调节细胞分化的因素是改善pullulan生产的关键.
研究的目的:
- 研究酸 (CA) 在调节Aureobasidium pullulans NG.NG.细胞分化中的作用.
- 为了确定CA对pullulan合成和生产产量的影响.
主要方法:
- 种植Aureobasidium pullulans NG,其中含有不同度的酸.
- 监测细胞分化,特别是胀细胞 (SC) 的流行情况.
- 通过已确定的方法量化普鲁兰生产.
主要成果:
- 观察到Aureobasidium pullulans NG细胞内的酸积累维持了胀的细胞 (SC) 形式.
- 与对照组 (10.23 g/l) 相比,添加1.0%的酸显著增加了pullulan产量,达到18.32 g/l.
- 由CA调节的细胞分化直接与增强的pullulan产量相关.
结论:
- 酸是Aureobasidium pullulans NG细胞分化的一个关键调节剂.
- 操纵细胞内CA水平支持SC形态,并增强pullulan生物合成.
- 酸补充剂是优化工业普鲁兰生产的可行策略.
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