基于aptamer的技术用于在土壤中检测细菌
Andrea Manfredini1, Eligio Malusà2,3, Loredana Canfora4
1CREA Centro di Ricerca Agricoltura e Ambiente, 00184, Rome, Italy. andrea.manfredini@crea.gov.it.
Applied microbiology and biotechnology
|September 12, 2023
概括
研究人员开发了用于在土壤中检测细菌细菌的DNA吸管,为监测生物菌剂和促进可持续农业提供了稳定且具有成本效益的DNA/RNA方法的替代方案.
科学领域:
- 微生物生态学 微生物生态学
- 生物技术是生物技术.
- 农业科学 农业科学
背景情况:
- 准确检测和跟踪在土壤中的微生物生物化剂对于评估它们对土壤质量和微生物群落的影响至关重要.
- 目前基于DNA/RNA的方法存在局限性,因此需要替代检测策略.
- 在生物传感应用中,DNA吸收剂提供了低成本,稳定性和易于修改等优势.
研究的目的:
- 为了隔离和表征单链DNA (ssDNA) 吸收体,用于细菌细菌的特定检测.
- 为了验证这些体在各种土壤类型和田间条件下检测B. subtilis的有效性.
- 建立阿普坦作为一种可行的方法来监测土壤中的微生物注射剂.
主要方法:
- 使用切换细胞的SELEX (通过指数式丰富对联体的系统进化) 方法来选择ssDNA的体.
- 使用动力参数 (Kd和Bmax) 进行了特征性阿巴胺亲和力和特异性.
- 在具有不同物理化学性质的土壤和现场试验中验证了aptamer性能.
主要成果:
- 确定了两种具有高亲属性 (纳米Kd) 和对B. subtilis菌株的特异性的ssDNA亚体.
- 在各种土壤矩阵和田间条件下成功验证了aptamer检测.
- 确认了aptamers适用于监测农业土壤中的B. subtilis注射剂的适用性.
结论:
- 开发了第一个能够将特定的Bacillus subtilis菌株结合到生物菌素配方中的DNA吸管.
- 首次成功地应用DNA吸收体来直接在土壤中检测细菌.
- 阿普塔默是一种有希望的,实用的,稳定的微生物注射剂检测方法,支持可持续农业和监管监督.
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