通过拉曼光谱学在鼻分泌物中诊断COVID-19:一项可行性研究
Ana Cristina Castro Goulart1, Renato Amaro Zângaro1,2, Henrique Cunha Carvalho2,3
1Universidade Anhembi Morumbi - UAM, R. Casa do Ator, 275, São Paulo, SP, 04546-001, Brazil.
Lasers in medical science
|September 12, 2023
概括
拉曼光谱为使用鼻分泌物诊断COVID-19提供了一种快速,无标签的方法. 这项技术通过分析病毒蛋白和宿主粘真菌特征,准确地区分感染和控制样本.
科学领域:
- 生物医学光谱学 生物医学光谱学
- 传染病诊断 传染病诊断 传染病诊断
- 分析化学 分析化学
背景情况:
- 由于COVID-19的流行,需要快速准确的诊断工具.
- 拉曼光谱已经显示出在血清中检测SARS-CoV-2的前景.
- 目前的研究探索其在鼻分泌中的应用.
研究的目的:
- 调查拉曼光谱法在从鼻分泌物诊断COVID-19的疗效.
- 确定分辨COVID-19阳性和阴性样本的光谱生物标志物.
- 开发一个用于COVID-19检测的分类模型.
主要方法:
- 从15名控制组和12名COVID-19阳性个体的鼻分泌物中获得了拉曼光谱.
- 使用一个分散式拉曼光谱仪 (830 nm,350 mW).
- 应用主要组件分析 (PCA) 和部分最小平方区分分析 (PLS-DA) 用于数据分析.
主要成果:
- 在COVID-19样本中,PCA确定了与SARS-CoV-2 N和S蛋白相关的光谱差异.
- 对照样本显示了与粘素成分 (氨酸,三氨酸,氨酸) 相关的特征.
- 一个PLS-DA模型实现了91%的准确性,80%的灵敏度和100%的特异性.
结论:
- 拉曼光谱可以有效地区分COVID-19患者与使用鼻分泌物的对照组.
- 该技术显示出作为COVID-19的快速,无标签的诊断方法的潜力.
- 谱分析揭示了关键的病毒和宿主相关生物标志物.
相关概念视频
01:26Raman Spectroscopy Instrumentation: Overview
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A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
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01:20Raman Spectroscopy: Overview
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The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...
443