在治疗经验丰富的印度安得拉邦患者中,Mycobacterium结核病分离的分子多样性
Anagoni Srikar1, Banda Venkataramana1, Alladi Mohan2
1Department of Microbiology, Sri Venkateswara Institute of Medical Sciences, Tirupati, Andhra Pradesh, India.
Journal of infection in developing countries
|September 12, 2023
概括
分子类型检测揭示了南印度患者的Mycobacterium结核复合体 (MTBC) 耐药性模式和遗传多样性. 北京菌株和异化单抗是值得注意的发现,突出了组合打字方法的实用性.
科学领域:
- 分子流行病学分子流行病学
- 传染病研究传染病研究.
- 结核菌菌的遗传学 结核菌的遗传学
背景情况:
- 了解Mycobacterium结核复合体 (MTBC) 传播和流行病学对于全球和当地结核病控制至关重要.
- 分子分类方法对于分析MTBC遗传多样性和种群结构至关重要.
- 以前接受治疗的肺结核患者是研究耐药性和传播动态的关键群体.
研究的目的:
- 为了研究MTBC的药物耐药性模式,先前在南印度接受治疗的,唾液涂抹阳性肺结核患者.
- 确定从该患者队列中分离的MTBC遗传多样性和种群结构.
- 评估用于MTBC表征的组合分子类型方法的实用性.
主要方法:
- 选择了104名先前接受过治疗的肺结核肺结核液涂抹阳性的患者队列.
- 进行药物敏感性测试以评估耐药性模式.
- 分子类型包括Spoligotyping,MIRU-VNTR和SNP类型,以分析遗传多样性和血统识别.
主要成果:
- 异化物单抗是最常见的,在15.38%的隔离物中观察到.
- 多药耐药 (MDR) 菌株占隔离物的23%.
- 占主导地位的MTBC家族包括EAI3-Ind (19.23%),EAI5 (12.50%) 和北京 (11.54%).
- 在MIRU-VNTR分析中,CAS1-德里和北京被确定为主要集团.
- 在SNP分析中,33个菌株的5组基因中没有突变.
结论:
- 北京菌株在印度南部的流行率为11.54%,这是一个重要的发现.
- 观察到异化单抗的高频率.
- 结合Spoligotyping,MIRU-VNTR和SNP类型,提供了一个强大的方法来识别MTBC菌株系.
- 在Antigen85C基因中缺乏突变表明疫苗开发的潜力.
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