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介质干细胞通过抑制线粒体质量控制相关的神经铁灭症来减轻脊髓损伤

Senyu Yao¹, Mao Pang², Yanheng Wang³

¹Department of Spine Surgery, The Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou, 510630, China; Center for Stem Cell Biology and Tissue Engineering, Key Laboratory for Stem Cells and Tissue Engineering, Ministry of Education, Sun Yat-Sen University, Guangzhou, 510275, China; National Medical Products Administration (NMPA) Key Laboratory for Quality Research and Evaluation of Cell Products, The Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou, 510630, China; Guangdong Engineering Technology Research Center of Minimally Invasive Spine Surgery, The Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou, 510630, China.

|September 12, 2023

概括

介质干细胞 (MSC) 可以通过转移线粒体,恢复线粒体质量控制和预防细胞死亡来抑制脊髓损伤 (SCI) 中的神经元铁亡. 这为中枢神经系统疾病提供了新的治疗方法.

科学领域:

神经科学是一个神经科学.
细胞生物学细胞生物学
生物医学工程生物医学工程

背景情况:

铁,一种依赖于铁的细胞死亡形式,有助于脊髓损伤 (SCI) 中的神经元损失.
线粒体功能障碍是SCI后神经元死亡的关键因素.
线粒体质量控制 (MQC) 对于神经元的生存至关重要,但其在铁亡中的作用尚不清楚.

研究的目的:

调查MQC在SCI后神经元铁亡中的作用.
阐明介质干细胞 (MSC) 保护神经元免受铁亡的机制.

主要方法:

单细胞RNA测序以确定SCI神经元中的ferroptosis.
分析线粒体动力学 (裂变和融合) 和线粒体细胞分裂.
介于介质干细胞 (MSC) 的线粒体转移通过道化纳米管.

主要成果:

在SCI中神经元主要经历铁亡,与失调的MQC,过度的线粒体分裂和线粒体衰变有关.
通过MSC介导的线粒体转移恢复神经元线粒体池并抑制铁亡.
细胞间道化纳米管促进了线粒体融合和铁灭的抑制.

结论:

在SCI中神经质亡是由MQC调节的.
通过通过细胞间线粒体转移改善线粒体健康,MSC减弱神经元铁亡.
基于MSC的线粒体疗法为涉及线粒体功能障碍的中枢神经系统疾病提供了潜在的治疗策略.

关键词:

细胞间的线粒体转移.介质细胞干细胞介质细胞干细胞线粒体质量控制的质量控制神经神经铁灭菌.脊髓损伤导致的脊髓损伤

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