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诱导的光激活在原生和突变的色光蛋白结合色光蛋白中

Gary C Jensen¹, Makena K Janis¹, Jazzmin Jara¹

¹Department of Chemistry, University of Houston, Houston, Texas 77204, United States.

|September 12, 2023

概括

菌染色衍生光蛋白在结合时显示增强的光. 这一发现使得新的基因编码金属离子传感器可以用于各种应用.

科学领域:

生物化学和分子生物学
光谱学和光学光学
蛋白质工程是指蛋白质的工程.

背景情况:

菌染色体 (CBCR) 衍生光蛋白是多功能的记者,在广泛的光谱中利用光共因子进行光.
这些源自蓝藻细菌的蛋白质通常具有GAF域,用于自催化蛋白结合和光.
来自Nostoc sp. 的All1280g2蛋白质. 一个含有DXCF动机的GAF域PCC7120,表现出独特的光化学反应,可以结合非光交换型基基 (PEB).

研究的目的:

为了研究All1280g2-PEB蛋白作为基因编码的金属离子传感器的潜力.
为了描述All1280g2-PEB中诱导的光变化.
阐明介导光增强背后的机制.

主要方法:

在离子 (Zn2+) 的存在下对All1280g2-PEB进行光谱分析.
针对All1280g2的局部导向突变发生,以探测特定残留物和对 bilin辅因子的固态接入的作用.
根据pH值进行光测量以确定解离常数.

主要成果:

所有1280g2-PEB在Zn2+结合时表现出可逆的~5倍光增强,具有蓝移排放最大值 (572到517纳米).
这种由诱导的增强在生物相关的pH范围 (6.0-9.0) 中观察到,具有不同的亲和力.
突变分析表明,DXCF基因中的一个氨酸残留物对诱导的光增强至关重要.

结论:

All1280g2-PEB 蛋白质具有独特的 Zn2+ 结合位点,可增强光,可能涉及与 bilin 辅因子和附近的氨酸的协调.
这种蛋白质作为开发新型,基因编码的金属离子光传感器的有希望的平台.
这些发现为CBCR衍生的蛋白质的结构功能关系及其在生物传感应用中的潜力提供了洞察力.

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