在室温下不同时间间隔发生在胰岛素中的结构变化的表征,通过表面增强的拉曼光谱学
Lubna Meraj1, Nasir Mehmood1, Muhammad Irfan Majeed1
1Department of Chemistry, University of Agriculture Faisalabad, Faisalabad 38000, Pakistan.
Photodiagnosis and photodynamic therapy
|September 12, 2023
概括
不适当的胰岛素储存降低了它的有效性,影响了血糖控制. 表面增强的拉曼光谱 (SERS) 能够有效检测胰岛素.
科学领域:
- 分析化学 分析化学
- 生物化学 生物化学
- 频谱学是一种光谱学.
背景情况:
- 在推温度 (FDA) 以上储存胰岛素会降解其蛋白质API,降低有效性和血糖控制.
- 蛋白质聚合与神经退行性疾病有关,如2型糖尿病,帕金森病和阿尔茨海默病.
- 表面增强拉曼光谱 (SERS) 显示了研究蛋白质变性化的潜力.
研究的目的:
- 用SERS和多变量分析分析室温储存期间液体制药胰岛素的结构变化.
- 为了评估胰岛素蛋白在室温下随着时间的推移而降解的情况.
主要方法:
- 银纳米粒子 (Ag-NPs) 被用作增强胰岛素光谱信号的SERS基材.
- 胰岛素的SERS光谱在初始冷藏后在室温下在不同时间间隔 (下午7点至早上7点) 收集.
- 多变量分析技术,包括主要成分分析 (PCA) 和部分最小平方回归分析 (PLSR),应用于光谱数据.
主要成果:
- 在室温储存后2小时内,SERS检测到胰岛素的结构变化.
- 基于储存时间,PCA成功地区分了胰岛素光谱,PC-1解释了77%的差异.
- PLSR证实了SERS的效率,提供了准确的储存时间预测,具有高的确定系数 (R2=0.98).
结论:
- SERS是一种高度敏感的技术,用于检测和区分胰岛素的结构变化.
- 这项研究表明,SERS能够在不理想的储存条件下监测胰岛素稳定性.
- 这一技术有助于确保胰岛素质量和患者安全.
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