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SHIELD:一种用于在人类细胞中对屏障类型DNA元素进行高通量选的平台

Meng Zhang^1,2, Mary Elisabeth Ehmann¹, Srija Matukumalli³

¹Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, USA.

Nature communications

|September 12, 2023

概括

研究人员开发了SHIELD,这是一个用于DNA边界元素高通量选的新平台. 这个工具可以从非编码基因组中识别出强大的屏障DNA元素,以实现稳定的转基因表达.

科学领域:

基因组学就是基因组学.
表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.
分子生物学分子生物学

背景情况:

染色体边界元素定义了基因组拓,并调节了基因表达.
来自边界元素的DNA绝缘体对于稳定的转基因表达至关重要.
由于缺乏选平台,对非编码边界元素的描述具有挑战性.

研究的目的:

开发一个高通量平台,用于选屏障类型的DNA元素.
从人类非编码基因组中发现具有绝缘特性的新型DNA元素.

主要方法:

开发了SHIELD (在拉米纳相关域的特定场所异性染色素插入元素) 进行选.
利用了氨酸整合酶的特异性,用于在面膜相关域 (LAD) 内的异染色素插入.
在人体细胞中选了1000个DNA元素的屏障活性.

主要成果:

确定了8种具有显著屏障活动的新型DNA元素.
鉴定出来的元素显示出与已确定的cHS4绝缘体核心区域相当的活性.
证明了SHIELD对DNA屏障元素的高通量评估的实用性.

结论:

盾牌是一个有效的平台,用于发现强大的屏障DNA元素.
从非编码基因组中可以使用SHIELD.识别出新的屏障元素.
这个平台有助于寻找稳定基因表达应用的新绝缘体.

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