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miRNA-126a在肌肉细胞和内皮细胞相互作用中起着重要作用
Bartosz Mierzejewski1, Maria Anna Ciemerych1, Wladyslawa Streminska1
1Department of Cytology, Faculty of Biology, University of Warsaw, Miecznikowa 1 St, 02-096, Warszawa, Poland.
Scientific reports
|September 12, 2023
概括
微RNAs调节肌肉干细胞与血管细胞的相互作用. miR-126a-3p抑制肌细胞迁移和融合,而miR-126a-5p促进血管形成.
科学领域:
- 肌肉干细胞生物学 肌肉干细胞生物学
- 分子生物学分子生物学
- 细胞相互作用 细胞相互作用
背景情况:
- 肌肉卫星细胞 (SCs) 对于骨肌肉再生至关重要.
- 利基中的SC-内皮细胞相互作用会影响SC命运.
- 微RNAs (miRNAs) 在这些SC-内皮细胞通信中的作用基本上是未知的.
研究的目的:
- 为了确定参与SC-内皮细胞交叉交谈的miRNAs.
- 研究已识别的miRNAs在SC行为和肌肉再生中的功能.
- 为了探索miRNAs对血管生成的影响.
主要方法:
- 由SC衍生的髓细胞被治疗以 stromal衍生因子-1 (SDF-1) 或使SDF-1受体 (CXCR4/CXCR7) 沉默.
- 进行了miRNA表达特征分析.
- 使用miRNA模拟器来研究特定miRNA对髓细胞迁移,融合和基因表达的影响.
- 用从转染的肌细胞中获得的条件介质来评估内皮细胞管原生.
主要成果:
- 确定了miR-126a-3p,并证明它可以抑制肌细胞迁移和融合.
- miR-126a-3p模仿了SDF-1和CXCR7的降低水平.
- miR-126a-3p模仿了肌肉发育过程中所涉及的减少因素.
- miR-126a-5p可以模仿增加的VEGF水平.
- 来自miR-126a-5p模仿转移的肌细胞的条件介质促进了内皮细胞管体生成.
结论:
- 特定的miRNAs,特别是miR-126a,在调节SC-内皮细胞相互作用方面发挥着重要作用.
- miR-126a-3p对肌细胞分化和与内皮细胞的通信产生负面影响.
- miR-126a-5p促进血管生成,这表明miR-126a在肌肉再生中的双重作用.
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