通过比较计算方法解读植物长非编码RNA的共享属性
Vikash Kumar Yadav1,2, Siddhi Kashinath Jalmi3, Shalini Tiwari4
1School of Biological Sciences and Biotechnology, Goa University, Taleigao Plateau, Goa, 403206, India. vikash.yadav@nipgr.ac.in.
Scientific reports
|September 12, 2023
概括
植物长非编码RNAs (lncRNAs) 在物种中显示保存的基因组和表观基因组特征. 这些由microRNAs (miRNAs) 调节的lncRNAs在植物生长和发育中起着至关重要的作用.
科学领域:
- 基因组学和表观基因组学
- 植物分子生物学 植物分子生物学
- 非编码RNA研究研究
背景情况:
- 长非编码RNAs (lncRNAs) 正在成为基因组功能的关键调节者.
- 了解lncRNA特征对于破译植物基因调节至关重要.
研究的目的:
- 在三个关键植物物种中描述lncRNAs的基因组和表观基因组特征:Arabidopsis thaliana,Oryza sativa和Zea mays.
- 研究lncRNA特征的保存及其与这些物种中的微RNA (miRNA) 和转录因子的相互作用.
主要方法:
- 对A. thaliana中的13,599个lncRNA,O. sativa中的11,565个和Z. mays中的32,397个的比较分析.
- 检查lncRNA分布,与可转移元素的关联,表观遗传标记 (H3K9me2,细胞因子甲基化) 和亚细胞局部.
- 识别保存的目标模仿位点,潜在的miRNA目标和转录因子结合亲缘关系.
主要成果:
- lncRNAs分布在染色体中,Helitron元素的丰富和它们的转录区域的终端倒置重复的耗尽.
- lncRNA转录区域表现出弱的表观遗传信号,除了H3K9me2和细胞因子甲基化.
- 确定了保存的miRNA-lncRNA相互作用,其中向基因参与了各种生物过程, lncRNAs对保存的转录因子如ERF和BBR-BPC表现出亲和力.
结论:
- 植物 lncRNAs 具有保留的基因组和表观基因组特征,这表明它们具有保留的调节作用.
- miRNA-lncRNA相互作用是一种影响植物基因表达和生物过程的保存机制.
- lncRNAs可能在不同物种的植物生长和发育中发挥着重要的,保存的作用.
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