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在2008年至2019年期间在荷兰国家监测期间收集的MRSA的分子表征

Leo M Schouls¹, Sandra Witteveen², Marga van Santen-Verheuvel²

¹Centre for Infectious Disease Control. National Institute for Public Health and the Environment (RIVM), Bilthoven, The Netherlands. leo.schouls@rivm.nl.

Communications medicine

|September 12, 2023

概括

荷兰对抗甲素的金黄色葡萄球菌 (MRSA) 的分子表征揭示了显著的基因组变化. 阳性MRSA的Panton-Valentine leucocidin (PVL) 的比例有所增加,这表明耐药性和毒性模式的演变.

科学领域:

分子流行病学分子流行病学
传染病监测传染病监测传染病监测传染病监测
细菌病原体的基因组分析

背景情况:

耐美西林黄金葡萄球菌 (MRSA) 在荷兰是一个重要的医疗保健挑战,尽管它的流行水平较低.
自1989年以来,国家MRSA监测一直在积极开展,以监测耐药性和毒性.
对MRSA分离物的分子表征对于了解种群动态至关重要.

研究的目的:

分析荷兰MRSA种群分子特征的时间变化.
为了确定MRSA菌株内新出现的耐药性和毒性基因配置文件.
研究MRSA的基因组多样性和传播.

主要方法:

分析了43,321个MRSA分离物 (2008-2019) 使用多个位置变量数量并列重复分析 (MLVA).
通过PCR检测mecA,mecC和Panton-Valentine leucocidin (PVL) 基因.
全基因组多部位序列类型化 (wgMLST) 和耐药性/病毒性基因识别使用来自4991个分离物的下一代测序数据.

主要成果:

具有PVL阳性的MRSA分离物的比例从15% (2008-2010) 增加到25% (2017-2019).
畜牧业相关的MRSA显示PVL阳性增加 (2017-2019年为6%),特别是来自较少养猪场的地区.
wgMLST确定了35个基因组,具有独特的个人资料,揭示了长时间的MRSA携带 (平均407天) 和区域菌株传播的证据.

结论:

大型MRSA队列的分子表征表明在国家一级显著的基因组进化.
该研究强调了MRSA类型和基因组的动态变化,包括PVL阳性菌株的增加.
基因组监测对于跟踪MRSA种群变化和为公共卫生战略提供信息至关重要.

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