一个通用战略向抗pH的基光体,用于高保真感应和生物成像应用
Sourav Sarkar1, Anushree Shil1, Suman Maity2
1Department of Chemistry, Pohang University of Science and Technology (POSTECH), Gyeongbuk, 37673, Republic of Korea.
Angewandte Chemie (International ed. in English)
|September 13, 2023
概括
研究人员开发了耐pH的光探针,通过将结接收器添加到类光体中. 这种简单的修改提高了稳定性和排放,使细胞和组织可靠的生物成像.
科学领域:
- 化学生物学 化学生物学
- 生物光子学 生物光子学
- 光成像技术 光成像技术
背景情况:
- 类光体对生物成像具有价值,但表现出pH取决于光的光.
- 这种pH敏感性限制了它们在pH变化的生物系统中的应用.
- 现有的研究在很大程度上忽视了对抗pH的基光体的需求.
研究的目的:
- 开发一种简单的方法来制造抗pH的基光体.
- 为了提高生物成像现有光体的光物理性质.
- 为了证明这些改性光体在细胞和组织成像中的实用性.
主要方法:
- 引入了一种结接纳体 (例如,形态素) 与基基组相邻.
- 合成的修改过的基化衍生物.
- 评估光物理性能,包括辐射光谱,稳定性和pH电阻.
- 在细胞和组织上进行了比度成像实验.
主要成果:
- 结构修改成功使基化物具有抗pH和"中等抗"的性能.
- 修改后的染料表现出巴托克罗姆辐射转移,增加了斯托克斯转移和增强的光稳定性.
- 在修改后的衍生品中,光强度显著提高.
- 细胞和组织的放射性成像显示出可靠的性能.
结论:
- 一个简单的结构修改可以将传统的基化物转化为强大的,抗pH的成像剂.
- 这一战略扩大了可用于准确可靠的生物成像的光体工具包.
- 增强的特性促进了先进的应用,如复杂的生物环境中的比度成像.
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