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构成性亚利碳化合物受体促进了小鼠骨髓中介细胞 stromal 细胞的再生潜力

Jing Huang¹, Yi-Ning Wang¹, Yi Zhou²

¹State Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology, School & Hospital of Stomatology, Wuhan University, Wuhan 430079, Hubei Province, China.

World journal of stem cells

|September 13, 2023

概括

亚利碳化合物受体 (AhR) 通过促进骨质生成和调节巨细胞,增强骨髓中细胞 (BMSCs) 的再生潜力. 这种效应部分通过增加信号传感器和转录3 (STAT3) 酸化激活器进行调解.

科学领域:

组织工程是组织工程.
细胞生物学细胞生物学
免疫学免疫学免疫学

背景情况:

骨髓中酶体 stromal 细胞 (BMSCs) 对于组织工程至关重要.
在BMSC中构成性阿里碳化合物受体 (AhR) 的作用尚不清楚.

研究的目的:

研究AhR在小鼠BMSCs (mBMSCs) 骨质生和巨调节能力中的功能.
阐明涉及AhR的潜在分子机制.

主要方法:

通过免疫化学和免疫光学来评估AhR表达.
通过性酸酶和阿利沙林红色染色来评估mbMSC骨质生成.
在与mBMSCs共同培养后,通过流细胞计分析了巨细胞的表型.
通过共免疫沉和西部斑点检查了AHR与STAT3和STAT3酸化的相互作用.

主要成果:

在小鼠骨髓和mBMSC中证实了AhR的表达.
过度表达AhR增强了mBMSC的骨质性潜力,而敲击抑制了它.
在mbMSCs中的AhR过度表达减少了促炎 (CD86+) 和增加了抗炎 (CD206+) 巨标志物.
AhR与STAT3直接相互作用,增强其酸化,从而部分调解AhR的作用.

结论:

在mBMSC的再生功能中,AhR起着积极的作用.
AhR增强了骨质分化,并促进了抗炎性巨细胞的特征.
艾哈的有益作用部分取决于STAT3酸化.

关键词:

阿里碳水化合物受体是什么?骨髓中细胞骨髓中细胞 stromal 细胞互动互动互动互动巨细胞是一个巨细胞.骨质发生过程 (osteogenesis)信号传感器和转录激活器 3 3

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