与结直肠癌瘤微环境相关的m6A调节者的异常遗传和表观遗传模式
Shuwei Ren1, Yanhong Xiao1, Huihui Wang1,2
1Department of Clinical Laboratory, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, China.
Translational cancer research
|September 13, 2023
概括
通过遗传和表观遗传变化,N6-甲基氨酸 (m6A) 调节器在结直肠癌 (CRC) 中经常发生变化. 了解这些m6A模式及其瘤微环境透是开发新型CRC免疫疗法的关键.
科学领域:
- 分子瘤学分子瘤学
- 癌症表观遗传学 癌症表观遗传学
- 免疫治疗是一种免疫疗法.
背景情况:
- N6-甲基氨酸 (m6A) 在癌症的发展和进展中至关重要.
- 结直肠癌 (CRC) 中m6A调节者的遗传,表观遗传和瘤微环境 (TME) 特性尚不清楚.
研究的目的:
- 为了研究CRC中m6A调节者的分子模式.
- 探索影响m6A调节器表达的遗传和表观遗传变化.
- 分析m6A调节器与CRC中的TME透之间的相关性.
主要方法:
- 使用了癌症基因组图谱 (TCGA) 数据,用于CRC样本中的24个m6A调节器.
- 评估突变,拷贝数变异 (CNVs),DNA甲基化和染色质可访问性.
- 使用瘤免疫估计资源 (TIMER) 来将m6A调节器与TME细胞透相关联.
主要成果:
- 大多数m6A调节器在CRC中受到失调,其中16个受上调,2个受下调.
- 在几个m6A调节器中观察到突变和CNV,副本数与表达正相关.
- 异常的DNA甲基化和高染色质可访问性是常见的,与基因表达相关;IGF2BP1作为一个独立的预后因素出现. 大多数调节剂与免疫细胞透 (B细胞,T细胞,巨细胞等) 有正相关. ) 的情况.
结论:
- 在CRC中异常的m6A调节器表达与遗传 (突变,CNV) 和表观遗传 (甲基化,染色质可访问性) 修改有关.
- 在TME中表征m6A调节器透,为开发向CRC免疫疗法提供了洞察力.
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