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稳定于FOLR1的β-catenin通过EGFR/AKT/GSK-3β通路促进喉癌的进展
Huawei Tuo1, Xuemei Li2, Haixia Du1
1Department of Otolaryngology, Taihe Hospital, Hubei University of Medicine, Shiyan, China.
Molecular carcinogenesis
|September 13, 2023
概括
叶酸受体1 (FOLR1) 通过稳定β-catenin,促进喉癌的生长. 这通过EGFR/AKT/GSK3β途径发生,突出了喉状细胞癌 (LSCC) 的新治疗标.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 细胞生物学 细胞生物学
背景情况:
- 叶酸受体1 (FOLR1) 在各种上皮癌中过度表达.
- 喉状细胞癌 (LSCC) 中FOLR1的特定作用尚不清楚.
- 了解FOLR1在LSCC中的功能对于开发向疗法至关重要.
研究的目的:
- 研究LSCC中FOLR1的表达和功能.
- 阐明FOLR1影响LSCC细胞行为的分子机制.
- 在LSCC中识别与FOLR1信号相关的潜在治疗点.
主要方法:
- 在LSCC组织与正常组织中对FOLR1mRNA和蛋白质表达的定量分析.
- 功能性测试评估LSCC细胞的增殖和迁移,有或没有FOLR1调制 (siRNA淘汰).
- 研究β-catenin的稳定性,无处不在和降解.
- 探索表皮生长因子受体 (EGFR) /AKT/糖原合成酶激酶3β (GSK3β) 信号轴的参与.
主要成果:
- 与相邻的正常组织相比,LSCC组织中的FOLR1表达 (mRNA和蛋白质) 显著增加.
- FOLR1增强了LSCC细胞的增殖和迁移;β-catenin的敲击取消了这些效应.
- FOLR1通过抑制其无处不在和降解来稳定β-catenin.
- 阻断EGFR/AKT/GSK3β通路抑制了FOLR1-介导对β-catenin表达和核转位的影响.
结论:
- 在促进LSCC进展方面,FOLR1发挥着重要作用.
- 通过稳定β-catenin,FOLR1增强了LSCC的扩散和迁移.
- EGFR/AKT/GSK3β信号通路调解FOLR1对β-catenin稳定性和核转位的影响,这代表了LSCC的潜在治疗策略.
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