时间分析显示,感觉和反感觉增强器RNA在协调免疫球蛋白羔羊激活中起着关键作用
Zeqian Gao1, Alastair L Smith1, James N F Scott1
1School of Molecular and Cellular Biology, Faculty of Biological Sciences, University of Leeds, Leeds LS2 9JT, UK.
Nucleic acids research
|September 13, 2023
概括
增强元件形成一个活跃的枢纽,协调基因段转录. 感觉增强器RNA积累增强了转录因子的结合,驱动了部位激活.
科学领域:
- 免疫学 免疫学 免疫学
- 分子生物学分子生物学
- 遗传学 遗传学 是一个
背景情况:
- 转录增强剂通过编排非编码转录来激活V(D) J重组.
- 在空间上不同的促进体中协调转录增长的机制尚不清楚.
研究的目的:
- 研究增强剂元素如何在小鼠免疫球蛋白兰巴 (Igλ) 位点协调转录.
- 阐明增强子RNA (eRNA) 在调节部位激活中的作用.
主要方法:
- 使用小鼠免疫球蛋白兰巴达 (Igλ) 位点作为模型系统.
- 进行了转录因子结合动态和增强剂元素相互作用的时间分析.
- 研究了感觉和反感觉增强器RNA之间的相互作用.
主要成果:
- 确定了三种类似增强剂的元素 (Eλ3-1,HSCλ1,HSE-1),形成了一个具有类似转录因子结合动态的活性增强剂枢纽.
- 观察到V和J基因段对增强器枢纽的协调招募.
- 证明早期事件包括E2A,p300,调解器和整合器结合,其次是YY1招募和eRNA合成.
- 表明反感觉Eλ3-1 eRNA抑制Igλ激活,而积累感觉eRNA促进YY1招募并稳定增强器枢纽/促进器相互作用.
结论:
- 建立了增强元件可以形成一个主动的枢纽,协调转录.
- 对于感觉与反感觉 eRNA 的值水平在调节位激活方面发挥了关键作用.
- 揭示了一个新的机制,感觉eRNA积累通过稳定增强剂-促进剂相互作用驱动转录激活.
更多相关视频
11:36Author Spotlight: An Integrated Workflow to Study the Promoter-Centric Spatio-Temporal Genome Architecture in Scarce Cell Populations
Published on: April 21, 2023
2.1K
08:12Assessing Somatic Hypermutation in Ramos B Cells after Overexpression or Knockdown of Specific Genes
Published on: November 1, 2011
20.0K
相关概念视频
RNA Polymerase II Accessory Proteins
9.2K
Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...
9.2K
Eukaryotic Transcription Activators
11.1K
Transcription activators are proteins that promote the transcription of genes from DNA to RNA. In most cases, these proteins contain two separate domains ‒ a domain that binds to DNA and a domain for activating transcription; however, in some cases, a single domain is responsible for both binding and activation of transcription, as seen in the glucocorticoid receptor and MyoD.
The binding domains are capable of recognizing and interacting with regulatory sequences on the DNA. These...
The binding domains are capable of recognizing and interacting with regulatory sequences on the DNA. These...
11.1K
Bacterial Transcription
28.4K
RNA polymerase (RNAP) carries out DNA-dependent RNA synthesis in both bacteria and eukaryotes. Bacteria do not have a membrane-bound nucleus. So, transcription and translation occur simultaneously, on the same DNA template.
Transcription can be divided into three main stages, each involving distinct DNA sequences to guide the polymerase. These are:
Transcription can be divided into three main stages, each involving distinct DNA sequences to guide the polymerase. These are:
28.4K
Inheritance of Chromatin Structures
6.3K
Epigenetics is the study of inherited changes in a cell's phenotype without changing the DNA sequences. It provides a form of memory for the differential gene expression pattern to maintain cell lineage, position-effect variegation, dosage compensation, and maintenance of chromatin structures such as telomeres and centromeres. For example, the structure and location of the centromere on chromosomes are epigenetically inherited. Its functionality is not dictated or ensured by the underlying...
6.3K
lncRNA - Long Non-coding RNAs
8.6K
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
8.6K
piRNA - Piwi-interacting RNAs
6.9K
PIWI-interacting RNAs, or piRNAs, are the most abundant short non-coding RNAs. More than 20,000 genes have been found in humans that code for piRNAs while only 2000 genes have been found for miRNAs. piRNAs can act at the transcriptional and post-transcriptional levels and have a vital role in silencing transposable elements present in germ cells. They are also involved in epigenetic silencing and activation. Previously, they were thought to function only in germ cells but new evidence suggests...
6.9K
