该Ustilago maydisAA10LPMO对真菌细胞壁素具有活性
Roseline Assiah Yao1, Jean-Lou Reyre1,2, Ketty C Tamburrini1,3
1INRAE, Aix Marseille Univ, UMR 1163 Biodiversité et Biotechnologie Fongiques (BBF), Marseille, France.
Applied and environmental microbiology
|September 13, 2023
概括
来自Ustilago的Lytic多糖胺单氧酶 (LPMOs) 可能会向真菌素,促进其被其他酶分解. 这项研究揭示了AA10 LPMOs在真菌细胞壁重塑中的新角色.
科学领域:
- 生物化学 生物化学
- 分子生物学分子生物学
- 菌类学 菌类学是指菌类学.
背景情况:
- 性多糖化单氧化酶 (LPMOs) 主要用于生物质转化.
- AA10 LPMOs在细菌,病毒和古生物中发现,对纤维素/酸盐活跃.
- AA10基因存在于致病性真菌中,如Ustilago maydis,其功能尚不清楚.
研究的目的:
- 来自Ustilago的AA10LPMO的特征可能是dis.
- 研究其对真菌素的活性及其在细胞壁重塑中的作用.
- 探索LPMOs除了生物质转化之外的其他角色.
主要方法:
- 在Pichia pastoris.cd中Ustilago maydis AA10 LPMO (UmAA10_cd) 的催化域的产生.
- 生物化学表征UmAA10_cd对基的活性.
- 测定UmAA10_cd与Ustilago maydis基因酶 (UmGH18A) 的协同作用.
主要成果:
- UmAA10_cd已成功生产并进行生化特征.
- UmAA10_cd表现出C1区域选择性氧化裂变的α-和β-胆.
- UmAA10_cd通过UmGH18A在真菌细胞壁上增强了基的水解.
结论:
- 来自Ustilago maydis的AA10LPMO对真菌细胞壁素具有活性.
- 这种LPMO在真菌生命周期期间在真菌细胞壁重塑中发挥作用.
- 在微生物环境中,真菌细胞壁是基活性LPMOs的潜在目标.
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