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相关概念视频

T Cell Types and Functions01:24

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When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
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T Cell Activation and Clonal Selection01:22

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T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
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TGF - β Signaling Pathway01:16

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The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...
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NF-κB-dependent Signaling Pathway02:26

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The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
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相关实验视频

Updated: Jul 16, 2025

Preparation of Single-Cell Suspension of Mouse Thymic Epithelial Cells and Staining of Intracellular Molecules for Flow Cytometric AnalysisMechanisms
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TGF-β促进IFN-γ-产生不变NKT细胞的选择后胸膜发育和外周功能.

Roxroy C Morgan1, Cameron Frank2, Munmun Greger2,3

  • 1Committee on Genetics, Genomics and Systems Biology, The University of Chicago, Chicago, IL.

Journal of immunology (Baltimore, Md. : 1950)
|September 13, 2023
PubMed
概括

转化增长因子-β (TGF-β) 信号传递对于发育和维护胸腺中不变的自然杀手T (iNKT1) 细胞的一个子集至关重要. 这一途径影响它们的基因表达和粘附分子呈现,突出显示组织特异性的iNKT1细胞调节.

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Evaluation of T Follicular Helper Cells and Germinal Center Response During Influenza A Virus Infection in Mice
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科学领域:

  • 免疫学 免疫学 免疫学
  • 细胞生物学 细胞生物学
  • 发展生物学 发展生物学

背景情况:

  • 不变的自然杀手T (iNKT) 细胞是与生俱来的类似的淋巴细胞,参与宿主防御.
  • 胸膜iNKT细胞在免疫调节中的特定作用尚未完全理解.
  • 胸膜iNKT细胞的子组影响各种免疫细胞群和胸膜上皮细胞.

研究的目的:

  • 调查转化生长因子-β (TGF-β) 信号传导在胸膜不变NKT1 (iNKT1) 细胞的发育和功能中的作用.
  • 为了识别特定的iNKT1细胞子集,这些子集依赖TGF-β进行发育和基因表达.
  • 将胸膜iNKT1细胞的TGF-β反应能力与外围组织的细胞进行比较.

主要方法:

  • 在小鼠胸膜iNKT1细胞中分析基因表达特征.
  • 细胞表面受体表达的评估,包括CD49a,CD103和CD69.
  • 来自胸腺,肝脏和脏的iNKT1细胞中的TGF-β信号通路的比较.

主要成果:

  • 一个小组的小鼠胸部iNKT1细胞需要TGF-β信号用于选择后的特定基因的发展和维护.
  • TGF-β信号传递对于这些iNKT1细胞上的粘附受体CD49a和CD103的表达至关重要,但不是CD69.
  • CD244+ c2 胸膜iNKT1 细胞,以 IFN-γ 生产和 NK 类特征而闻名,是这种 TGF-β 响应群体的一部分.
  • 肝脏和脏iNKT1细胞缺乏在胸膜iNKT1细胞中发现的特定TGF-β基因特征,尽管TGF-β仍然影响它们的表型和功能.

结论:

  • TGF-β信号传递在发育和维护特定的胸膜iNKT1细胞子集中起着至关重要的作用.
  • 这项研究揭示了iNKT1细胞发育机制在不同组织中的异质性.
  • 胸膜iNKT1细胞的子集与TGF-β产生细胞之间存在密切的关联,支持它们的发育.