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非正规的Rab9a作用支持人类乳头瘤病毒在病毒进入过程中通过逆转激素介导的内体外出

Jeongjoon Choi¹, Daniel DiMaio^1,2,3,4

¹Department of Genetics, Yale School of Medicine, New Haven, Connecticut, United States of America.

|September 13, 2023

概括

人类乳头瘤病毒 (HPV) 的进入被GTP结合的Rab9a意外地抑制,Rab9a是囊泡传输的关键调节者. 这项研究揭示了Rab9a贩运机械的独特病毒和细胞用途.

科学领域:

细胞生物学细胞生物学
病毒学病毒学
分子贩运分子贩运行为

背景情况:

拉布GTPases对于细胞内囊泡运输至关重要,与GTP结合的形式促进囊泡运动.
逆转酶是一种参与逆向运输的蛋白质复合体,对于从内分体到戈尔吉装置的运输货物至关重要.

研究的目的:

调查Rab9a在人类乳头瘤病毒 (HPV) 细胞内贩运和进入中的作用.
阐明HPV在病毒进入过程中与复原体和Rab9a相互作用的机制.

主要方法:

在宿主细胞中抑制Rab9a的表达.
使用生物化学测定对HPV-逆转子相互作用的分析.
显微镜来追踪HPV局部化和内分泌体中的积累.
操纵Rab9a GTP/GDP负载以评估其对HPV入境的影响.

主要成果:

与GTP结合的Rab9a抑制了复原体介导的HPV传递到逆行途径.
Rab9a knockdown通过改变HPV-复原体协会和阻断内分体到戈尔吉传输来破坏HPV的进入.
在感染初期,HPV与Rab9a相互作用,独立于Rab7,而Rab9a的独特的GTP/GDP-bound状态对HPV的进入有差异性影响.

结论:

HPV 使用 Rab9a 主体贩运机器的方式与细胞蛋白质货物不同.
Rab9a与GTP结合的形式作为HPV进入的抑制剂,表明其具有复杂的调节作用.
了解这些独特的贩运机制,可以了解病毒进入策略.

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