由支柱构建的L-ribose特定识别表面[5]以arene为基础的宿主-客人相互作用.
Wenhui Liu1, Weiwei Xu2, Hang-Hang Luan3
1College of Chemistry, Huazhong Agricultural University, Wuhan, Hubei, 430070, PR China.
Biosensors & bioelectronics
|September 13, 2023
概括
研究人员开发了一种奇拉黄金表面,使用宿主-客人相互作用来检测L-ribose. 这种功能界面显示出良好的检测能力和可回收性,将识别与智能结合起来.
科学领域:
- 生物化学 生物化学
- 材料科学 材料科学 材料科学
- 分析化学 分析化学
背景情况:
- 状分子根据它们的形状表现出明显的生理效应.
- 核糖是RNA的组成部分,对于调节生物活动至关重要.
- 生物糖识别机制激发了性感应平台的发展.
研究的目的:
- 构建功能性合面,通过非共价相互作用识别L-ribose.
- 为了创建一个黄金表面功能化与宿主-客分子的L-ribose检测.
- 将接口识别与智能响应能力相结合.
主要方法:
- 在金色表面上组装单一功能基柱[5]arene (SAP5) 作为主体和 (S) -mandelate-violet (SMV) 作为客人.
- 使用关联常数和绑定比率进行主机-客户互动分析.
- 使用阻抗和接触角度测量检测L-ribose.
- 通过将复合物用粉拆卸来证明可回收性.
主要成果:
- 通过使用SAP5和SMV,成功构建了一个功能性形黄金表面.
- 确定SMV和SAP5之间的关联常数为2.95×10^4 M^-1,具有1:1的结合比.
- 开发的接口有效地检测到L-ribose在1×10^-7 M到1×10^-2 M的度范围内.
- 该系统表现出良好的可回收性,识别复合体被拆卸并可重复使用.
结论:
- 这项研究成功地创建了一个能够通过宿主-客人相互作用识别L-ribose的奇拉黄金表面.
- 开发的传感器具有对L-ribose的敏感检测和良好的可回收性.
- 接口识别与智能拆卸的整合为生物传感应用提供了一种新的方法.
相关概念视频
02:35Noncovalent Attractions in Biomolecules
51.8K
Noncovalent attractions are associations within and between molecules that influence the shape and structural stability of complexes. These interactions differ from covalent bonding in that they do not involve sharing of electrons.
Four types of noncovalent interactions are hydrogen bonds, van der Waals forces, ionic bonds, and hydrophobic interactions.
Hydrogen bonding results from the electrostatic attraction of a hydrogen atom covalently bonded to a strong-electronegative atom like oxygen,...
Four types of noncovalent interactions are hydrogen bonds, van der Waals forces, ionic bonds, and hydrophobic interactions.
Hydrogen bonding results from the electrostatic attraction of a hydrogen atom covalently bonded to a strong-electronegative atom like oxygen,...
51.8K
01:24Oligosaccharide Assembly
2.9K
Protein glycosylation starts in the ER lumen and continues in the Golgi apparatus. Glycosyltransferases catalyze the addition of sugar molecules or glycosylation of proteins. Usually, these enzymes add sugars to the hydroxyl groups of selected serine or threonine residues to form O-linked glycans or the amino groups of asparagine residues to form N-linked glycans. Different positions on the same polypeptide chain can contain differently linked glycans.
Multiple sugar molecules that may or may...
Multiple sugar molecules that may or may...
2.9K
01:01Phosphodiester Linkages
100.4K
Overview
Phosphodiester bond forms when a phosphoric acid molecule (H3PO4) links with two hydroxyl groups (–OH) of two other molecules, forming two ester bonds. Two water molecules are released in this process. The phosphodiester bond is commonly found in nucleic acids (DNA and RNA) and plays a critical role in their structure and function.
Phosphodiester Bonds Link Nucleotides Together
DNA and RNA are polynucleotides or long chains of nucleotides that are linked together. A nucleotide is...
Phosphodiester bond forms when a phosphoric acid molecule (H3PO4) links with two hydroxyl groups (–OH) of two other molecules, forming two ester bonds. Two water molecules are released in this process. The phosphodiester bond is commonly found in nucleic acids (DNA and RNA) and plays a critical role in their structure and function.
Phosphodiester Bonds Link Nucleotides Together
DNA and RNA are polynucleotides or long chains of nucleotides that are linked together. A nucleotide is...
100.4K
01:14Glycocalyx and its Functions
4.1K
The glycocalyx is a carbohydrate-rich, fuzzy-appearing layer on the outer surface of the cell membrane. It is highly hydrophilic, because of this it attracts large amounts of water to the cell's surface. This aids the cell's interaction with the watery environment and also helps it to obtain substances dissolved in the water. It is also important for cell identification, self/non-self determination, and embryonic development and is used in cell-to-cell attachments to form tissues.
4.1K
01:30Membrane Carbohydrates
5.5K
The plasma membrane is a dynamic barrier composed of lipids, proteins, and carbohydrates. It is the epicenter of many cellular processes required for cell growth and survival. Carbohydrates have unique structural and chemical properties that help the plasma membrane to carry out its functions effectively.
Membrane carbohydrates do not have any hydrophobic region and are exclusively located on the cell's outer surface. The addition of sugar molecules or glycosylation of proteins happens in...
Membrane carbohydrates do not have any hydrophobic region and are exclusively located on the cell's outer surface. The addition of sugar molecules or glycosylation of proteins happens in...
5.5K
01:23RNA Structure
71.6K
Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
71.6K