阿拉比多普西斯的转录因子WRKY45通过激活PCS1和PCS2表达来赋予耐受性
Fangjian Li1, Yaru Deng1, Yan Liu1
1Root Biology Center, South China Agricultural University, Guangzhou 510642, China; College of Natural Resources and Environment, South China Agricultural University, Guangzhou 510642, China.
阿拉比多普西斯的转录因子WRKY45通过促进植物素 (PC) 合成来增强的耐受性. WRKY45激活PCS1和PCS2基因表达,导致PC积累增加和植物中的毒性降低.
科学领域:
- 植物生物学 植物生物学
- 环境毒理学环境毒理学
- 分子遗传学 分子遗传学
背景情况:
- (Cd) 是一种有毒的污染物,在全球范围内影响农作物.
- 植物 (PC) 生物合成对于植物排毒至关重要.
- PC生物合成的调节机制在很大程度上仍然是未知的.
研究的目的:
- 阐明阿拉比多普西斯转录因子WRKY45在耐受性中的作用.
- 研究WRKY45如何影响PC合成基因的表达.
- 了解WRKY45赋予抗性的分子机制.
主要方法:
- 在应力下分析WRKY45表达.
- 对WRKY45突变体和过度表达的植物进行表型分析.
- 定量实时PCR用于评估AtPCS1和AtPCS2表达.
- 电泳移动转移试验 (EMSA) 用于确定WRKY45与PCS2促进体结合.
- 测量PC积累的方法.
主要成果:
- 应激增加了WRKY45转录和蛋白质水平的调节.
- wrky45突变体表现出增加的敏感性,而WRKY45过度表达者表现出增强的耐受性.
- WRKY45过度表达刺激了AtPCS1和AtPCS2的表达,而wrky45突变显示表达减少.
- WRKY45直接与PCS2促进体中的W-box cis元素结合.
- 过度表达WRKY45会增加PC积累,而PCS1/PCS2过度表达会拯救wrky45突变表型.
结论:
- 阿拉比多普西斯 WRKY45 是耐受性的积极调节者.
- WRKY45通过激活PC合成基因PCS1和PCS2.2的表达来促进耐受性.
- 这些发现揭示了在涉及WRKY45.5的工厂中排毒的关键监管途径.
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