通过调节自,RhoB促进了沙门氏菌的生存
Marco Kirchenwitz1, Jessica Halfen1, Kristin von Peinen1
1Department of Cell Biology, Helmholtz Centre for Infection Research, Inhoffenstrasse 7, 38124 Braunschweig, Germany.
European journal of cell biology
|September 13, 2023
概括
沙门氏菌 Typhimurium 感染上调RhoB,一个小的GTPase 与效应器SopB相互作用. RhoB促进细菌的生存和自,对沙门氏菌至关重要.
科学领域:
- 微生物学 微生物学
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
背景情况:
- 沙门氏菌Typhimurium利用Sope,Sope2和SopB等III型分泌系统 (T3SS) 效应器来操纵宿主Rho GTPases (Rac1,Cdc42,Rhoa),从而促进细菌的入侵.
- 这些因子对S. Typhimurium的入侵及其细胞内生活方式至关重要,影响感染的早期和后期阶段.
研究的目的:
- 研究小GTPase RhoB在沙门氏菌Typhimurium入侵和细胞内生存中的作用.
- 为了阐明RhoB,沙门氏菌效应物SopB和宿主细胞过程 (如自和真空逃逸) 之间的关系.
主要方法:
- 在沙门氏菌感染期间RhoB表达的定量分析.
- 同免疫沉测试以确定SopB-RhoB相互作用.
- 同焦显微镜用于追踪含沙门氏菌的真空孔上的RhoB定位.
- 在野生型和突变型沙门氏菌株中评估细菌生存,真空逃生和自诱导.
主要成果:
- 沙门氏菌感染显著上调RhoB表达,这取决于SopB的效应因子,而不是其酸酶活性.
- SopB和RhoB相互作用,RhoB定位到含有细胞内S. Typhimurium的早期菌体中.
- SopB和RhoB都增强了沙门氏菌的细胞内生存率;RhoB特别需要用于沙门氏菌诱导的自细胞上调.
- 缺少RhoB会减少沙门氏菌的真空逃生和随后的细胞复制,这表明它对细胞内增殖的重要性.
结论:
- 在Salmonella Typhimurium的细胞内生活方式中,RhoB发挥着重要作用,特别是通过自促进细菌生存.
- 一个积极的反循环存在,沙门氏菌效应物SopB促进RhoB介导的自,增强细菌细胞内持久性.
- 准SopB-RhoB相互作用或RhoB介导的自可能是对抗沙门氏菌感染的新策略.
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