瘤向siRNA通过特定于腺素受体的curdlan纳米粒子传递
Qingming Bao1, Tsogzolmaa Ganbold1, Mingming Bao1
1Inner Mongolia Key Laboratory of Mongolian Medicinal Chemistry, School of Chemistry & Chemical Engineering, Inner Mongolia University, Hohhot, Inner Mongolia 010020, PR China.
International journal of biological macromolecules
|September 13, 2023
概括
化腺功能化氨基库德兰 (pAVC) 聚合物增强了核酸药物的生物相容性和瘤向性. 这些pAVC聚合物特别将siRNA传递给瘤,通过击倒STAT3.3来抑制生长.
科学领域:
- 生物材料科学 生物材料科学
- 纳米技术 纳米技术
- 药物运输 药物运输 药物运输
背景情况:
- 氨基化库德兰衍生物显示出作为核酸载体的潜力.
- 接体功能化兰衍生物通过受体介导内细胞分裂增强细胞特异性吸收.
- 提高生物相容性和瘤向性对于有效的核酸药物输送至关重要.
研究的目的:
- 开发基化腺功能化氨基库德兰 (pAVC) 聚合物,以提高生物相容性和瘤向性.
- 评估pAVC聚合物在输送核酸药物中的瘤向疗效和安全性.
- 为了确认pAVC聚合物的腺受体 (AR) 依赖的细胞吸收机制.
主要方法:
- 化腺功能化氨基库德兰 (pAVC) 聚合物的合成和表征.
- 在pAVC聚合物介导siRNA输送和瘤细胞中细胞毒性的体外评估.
- 在体内评估pAVC-siRNA纳米颗粒的瘤向效率和治疗效果.
- 使用特定抑制剂 (AMP,GMP) 确认腺受体 (AR) 的依赖性.
主要成果:
- pAVC聚合物表明瘤细胞吸收AR-依赖的siRNA,被AMP抑制.
- 与非化衍生物相比,pAVC聚合物表现出保存的受体识别和显著降低的细胞毒性.
- pAVC纳米颗粒在体内显示出显著的瘤向效率.
- 通过pAVC4聚合物 (最高PEG替代) 传递的siRNA专门针对瘤组织,导致STAT3敲击和瘤生长抑制.
结论:
- pAVC聚合物是一种有希望的,生物相容的载体系统,用于向的核酸药物输送.
- 开发的pAVC聚合物增强了siRNA的瘤特异性传递和治疗疗效.
- 这一策略为使用基于核酸的药物治疗癌症提供了潜在的进步.
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