Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Mass Analyzers: Common Types01:19

Mass Analyzers: Common Types

642
The quadrupole mass analyzer consists of four cylindrical metal rods arranged in a diamond carrying a DC voltage and a radio-frequency AC voltage. The motion of ions through the quadrupole depends on the field strength, causing only ions of a certain m/z to resonate successfully and strike the detector at a given field strength. Though the transmission rate for these analyzers is high, the exact elemental composition of the sample is not determined because of low resolution; however, they are...
642

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

AlignPCA-2D: PCA-reduced Euclidean vector alignment for 2D classification in cryo-EM.

Acta crystallographica. Section D, Structural biology·2026
Same author

Rational acquisition of laboratory equipment: an accurate mathematical model to estimate the trade-offs in shared and nonshared equipment.

Acta crystallographica. Section D, Structural biology·2026
Same author

Annexin A2 stabilizes the endoplasmic reticulum and actin cytoskeleton and influences the formation of reovirus factories.

Journal of virology·2025
Same author

How many (distinguishable) classes can we identify in single-particle analysis?

Acta crystallographica. Section D, Structural biology·2025
Same author

Automatic detection of alignment errors in cryo-electron tomography.

Journal of structural biology·2024
Same author

Real-space heterogeneous reconstruction, refinement, and disentanglement of CryoEM conformational states with HetSIREN.

bioRxiv : the preprint server for biology·2024
Same journal

Ultrastructural evidence of autophagy-related processes and mitochondrial remodeling in the myxozoan parasite Henneguya piaractus.

Journal of structural biology·2026
Same journal

Architecture and dynamics of a supramolecular oxygen transport system in human homogentisate 1,2-Dioxygenase.

Journal of structural biology·2026
Same journal

Connecting pathways between mineralized fibrocartilage and bone at the Achilles tendon insertion.

Journal of structural biology·2026
Same journal

Structural and functional characterization of thermostable EstS1 esterase for BHET degradation.

Journal of structural biology·2026
Same journal

Following the white rabbit: multiscale 2D3D correlative imaging of bone structure.

Journal of structural biology·2026
Same journal

The mantis shrimp eye imaged in 3D using 4th generation synchrotron multiscale phase contrast tomography.

Journal of structural biology·2026
查看所有相关文章

相关实验视频

Updated: Jul 16, 2025

Mass-Sensitive Particle Tracking to Characterize Membrane-Associated Macromolecule Dynamics
13:30

Mass-Sensitive Particle Tracking to Characterize Membrane-Associated Macromolecule Dynamics

Published on: February 18, 2022

4.5K

一个新的粒子加权减法算法,以减少来自不需要的元件的信号,用于单颗粒子分析.

E Fernández-Giménez1, M M Martínez2, R Marabini1

  • 1Centro Nac. Biotecnología (CSIC), c/Darwin, 3, 28049 Cantoblanco, Madrid, Spain; Univ. Autonoma de Madrid, 28049 Cantoblanco, Madrid, Spain.

Journal of structural biology
|September 13, 2023
PubMed
概括
此摘要是机器生成的。

一种新的投射减法方法增强了冷电子显微镜 (cryo-EM) 中的单颗粒分析 (SPA),以获得更清晰的宏分子结构. 这种先进的技术提高了高分辨率地图的生成,即使是复杂的样本与多个组件.

关键词:
化电磁波是一种冷电磁波.连接物 连接物 连接物纳米光盘是一个纳米盘.投影减去减去的投影.这是一个SPA SPA.

更多相关视频

AMEBaS: Automatic Midline Extraction and Background Subtraction of Ratiometric Fluorescence Time-Lapses of Polarized Single Cells
06:03

AMEBaS: Automatic Midline Extraction and Background Subtraction of Ratiometric Fluorescence Time-Lapses of Polarized Single Cells

Published on: June 23, 2023

497
Author Spotlight: Enhancing Cryo-EM Sample Preparation with Streptavidin-Biotin Approach
04:51

Author Spotlight: Enhancing Cryo-EM Sample Preparation with Streptavidin-Biotin Approach

Published on: December 29, 2023

5.6K

相关实验视频

Last Updated: Jul 16, 2025

Mass-Sensitive Particle Tracking to Characterize Membrane-Associated Macromolecule Dynamics
13:30

Mass-Sensitive Particle Tracking to Characterize Membrane-Associated Macromolecule Dynamics

Published on: February 18, 2022

4.5K
AMEBaS: Automatic Midline Extraction and Background Subtraction of Ratiometric Fluorescence Time-Lapses of Polarized Single Cells
06:03

AMEBaS: Automatic Midline Extraction and Background Subtraction of Ratiometric Fluorescence Time-Lapses of Polarized Single Cells

Published on: June 23, 2023

497
Author Spotlight: Enhancing Cryo-EM Sample Preparation with Streptavidin-Biotin Approach
04:51

Author Spotlight: Enhancing Cryo-EM Sample Preparation with Streptavidin-Biotin Approach

Published on: December 29, 2023

5.6K

科学领域:

  • 结构生物学是结构生物学.
  • 生物物理学的生物物理.
  • 生物化学 生物化学

背景情况:

  • 用冷电子显微镜 (cryo-EM) 进行单颗粒分析 (SPA) 对于确定生物大分子的近原子结构至关重要.
  • 现有的方法可以实现许多样本的高分辨率,但在涉及多个灵活子单元,病毒体或纳米光盘等稳定剂的复杂案例中扎.

研究的目的:

  • 开发一种新的,更精确的投影减法方法,用于冷-EM SPA.
  • 为了提高当前最先进的投影减法技术的性能.

主要方法:

  • 实现了一个新的投影减法算法.
  • 使用公共数据库和内部数据集测试算法.
  • 结果与现有的投影减法方法进行比较.

主要成果:

  • 开发的算法在冷EM SPA中显示出比现有方法更好的性能.
  • 在复杂的生物样本中提升了不需要的成分的信号去除.
  • 成功地定位了小联结体,如药物,具有以前未知的结合点.

结论:

  • 新的投影减法方法为冷EM SPA.提供了显著的进步.
  • 它可以实现更高分辨率的结构确定,特别是对于具有挑战性的样本.
  • 该方法有助于识别连接体结合点,有助于药物发现工作.