阿拉比多普西斯的TCP4转录因子抑制了高温诱导的卵子的同源转化
Jingqiu Lan1,2, Ning Wang1, Yutao Wang1
1State Key Laboratory of Protein and Plant Gene Research, School of Life Sciences, Peking University, Beijing, 100871, China.
Nature communications
|September 13, 2023
概括
高温威胁着植物,但II类TEOSINTE分支1/CYCLOIDEA/PCF (TCP) 转录因子保护了卵子的身份. 这项研究揭示了它们在热应激下维持植物生殖结构的关键作用.
科学领域:
- 植物生物学 植物生物学
- 分子遗传学 分子遗传学
- 环境压力反应环境压力反应
背景情况:
- 全球变暖导致高温 (HT),威胁到植物的生存和粮食安全.
- 对于HT对植物器官认同,特别是卵子发育的影响,仍然不太清楚.
研究的目的:
- 调查II类TEOSINTE分支1/CYCLOIDEA/PCF (TCP) 转录因子在高温下维持卵子身份中的作用.
- 阐明了植物生殖器官发育中由热引起的变化背后的分子机制.
主要方法:
- 对第二类TCP转录因子的双双偶突变 (tcpDUO) 的分析.
- 使用TCP4表达式的补充实验.
- 涉及TCP4,AGAMOUS (AG),SEPALLATA3 (SEP3) 和BELL1 (BEL1) 的蛋白质与蛋白质相互作用研究.
- 基因相互作用分析与卵子身份基因BELL1 (BEL1) 和STK.
主要成果:
- 这种tcpDUO突变体表现出HT诱导的卵子转化为carpelloid结构.
- 在tcpDUO突变体中,TCP4表达拯救了卵子身份转换表型.
- 发现TCP4与AG,SEP3和BEL1相互作用,增强了BEL1-AG-SEP3复合体.
- 这种tcpDUO突变与bel1和stk突变发生了协同的基因相互作用,进一步破坏了卵子的身份.
结论:
- 第二类TCP转录因子在保护卵子身份免受高温影响方面发挥了冗余和关键的作用.
- 这些TCP因素将内部遗传通路与环境温度线索相结合,以维持生殖器官的发育.
- 这些发现提供了关于植物适应气候变化的分子基础的见解,在器官身份层面.
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