单细胞同位素追踪揭示了与藻Phaeodactylum tricornutum相关的细菌的功能性协会
Xavier Mayali1, Ty J Samo2, Jeff A Kimbrel2
1Physical and Life Sciences Directorate, Lawrence Livermore National Laboratory, Livermore, CA, USA. mayali1@llnl.gov.
Nature communications
|September 13, 2023
概括
藻类有机物质的细菌再矿化促进了藻类的生长,但很少得到量化. 我们确定了三个不同的细菌功能学会,揭示了菌株特定的营养循环,这对于藻类生长预测至关重要.
科学领域:
- 海洋微生物生态学
- 生物地质化学循环过程
背景情况:
- 藻类有机物质的细菌再矿化对于初级生产至关重要.
- 在细菌和藻类之间量化营养转移仍然具有挑战性.
- 很难预测特定细菌种群的营养供应.
研究的目的:
- 量化细菌对藻类有机碳和的吸收.
- 测量藻类对再矿化营养素的吸收.
- 为了确定参与营养循环的功能性细菌协会.
主要方法:
- 使用稳定的同位素进行单细胞同位素追踪.
- 纳米SIMS (纳米级二次离子质谱) 用于元素映射.
- 与十五种细菌菌株一起共同培养藻类Phaeodactylum tricornutum.
主要成果:
- 在营养物质的纳入方面,有明显的菌株对菌株和细胞对细胞的变异性.
- 复杂有机的不均利用和重矿化.
- 识别了三个功能性协会:宏分子重矿化剂,宏分子使用者和小分子使用者.
- 小分子使用者在低碳条件下表现出高效增长.
结论:
- 细菌的功能性协会并不严格地与分化学有关.
- 来自基因组学的代谢能力预测不能完全捕捉到现场活动.
- 直接活动测量对于理解微生物代谢相互作用和营养循环至关重要.
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