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相关概念视频

Cryo-electron Microscopy01:28

Cryo-electron Microscopy

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Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
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Protein Dynamics in Living Cells01:19

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Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
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Ultrathin liquid cells for microsecond time-resolved cryo-EM.

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相关实验视频

Updated: Jul 16, 2025

Correlative Microscopy for 3D Structural Analysis of Dynamic Interactions
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通过微秒时间解析的冷EMEM揭示了快速的病毒动态.

Oliver F Harder1, Sarah V Barrass1, Marcel Drabbels1

  • 1Ecole Polytechnique Fédérale de Lausanne (EPFL), Laboratory of Molecular Nanodynamics, CH-1015, Lausanne, Switzerland.

Nature communications
|September 13, 2023
PubMed
概括

微秒时间分辨率的冷电子显微镜 (cryo-EM) 现在允许直接观察快速蛋白质动态. 这一突破揭示了牛菌性斑点病毒 (CCMV) 囊体变化的快速机制,进步了我们对蛋白质功能的理解.

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Method for Measurement of Viral Fusion Kinetics at the Single Particle Level
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科学领域:

  • 结构生物学 结构生物学
  • 生物物理学的生物物理.
  • 病毒学 病毒学

背景情况:

  • 观察蛋白质的作用对于理解它们的功能至关重要,但受到技术限制的限制.
  • 目前的方法缺乏时间分辨率来捕捉快速的蛋白质动态.

研究的目的:

  • 为了证明微秒时间分辨率冷电子显微镜 (cryo-EM) 观测快速蛋白质动态的能力.
  • 为了阐明牛类性斑点病毒 (CCMV) 体形状变化的机制.

主要方法:

  • 开发和应用微秒时间分辨率的冷EM.
  • 通过pH跳跃诱导CCMV的快速形状变化.
  • 高分辨率成像以捕捉动态事件.

主要成果:

  • 在pH值变化时观察到CCMV体在微秒时间尺度上的收缩.
  • 标志着囊蛋白的协调而又多个时间尺度的运动.
  • 揭示了形状变化的曲线反应路径.

结论:

  • 微秒时间解析的冷EM是一种强大的技术,用于研究快速蛋白质动态.
  • 这项研究为CCMV体机制提供了前所未有的洞察力.
  • 这种技术为研究广泛的以前无法观察到的蛋白质动态开辟了新的途径,促进了对蛋白质功能的基本理解.