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一种基于光的新方法,用于直接可视化Bacillus cereus中胞形成期间的外层形成

Armand Lablaine^1,2, Stéphanie Chamot¹, Mónica Serrano³

¹INRAE, Avignon Université, UMR SQPOV, 84000, Avignon, France.

Scientific reports

|September 13, 2023

概括

现在可以使用超分辨率显微镜在活细胞中可视化Bacillus cereus子外套的形成. 这项技术揭示了,即使在分泌过程中吞被阻止时,外套材料和一个关键蛋白质也会积聚.

科学领域:

微生物学微生物学
细菌子的形成
细胞成像细胞成像

背景情况:

麦芽菌,炭菌和土灵菌形成了具有蛋白质外衣和外的子,对环境相互作用和发芽至关重要.
传统的对子外和外形成的可视化利用固定细胞上的传输电子显微镜.
超分辨率显微镜 (SR-SIM) 已经使B. cereus.中的活细胞对外组合的可视化成为可能.

研究的目的:

使用超分辨率显微镜可视化活细胞中Bacillus cereus子外衣形成的不同步骤.
为了描述在低于最佳温度下形成的吞缺陷的B. cereus细胞.
为了研究外层形成,外蛋白合成和吞进展之间的关系.

主要方法:

超分辨率结构化照明显微镜 (SR-SIM) 用于活细胞成像.
使用MitoTrackerGreen (MTG) 和SNAP细胞TMR星染料作为光标记物.
在低于最佳的化温度下,化缺陷的B. cereus细胞子群的表征.

主要成果:

使用MTG和SNAP细胞TMR恒星染料的SR-SIM成功地可视化了活细胞中的Bacillus cereus子外衣形成步骤.
在低于最佳的分泌温度下,发现了吞缺陷细胞的亚群.
观察到在吞过程中被捕的细胞中合成和积累的外衣物质和s^K依赖蛋白BxpB.

结论:

活细胞SR-SIM是一种可行的方法,可用于可视化Bacillus cereus子外套组装.
子外层材料和BxpB合成并不严格依赖于B. cereus.中子吞的完成.
这与Bacillus subtilis形成鲜明对比,这表明这些物种间的胞调节有差异.

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