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Updated: Jul 16, 2025

Isolation and Time-Lapse Imaging of Primary Mouse Embryonic Palatal Mesenchyme Cells to Analyze Collective Movement Attributes

Isolation and Time-Lapse Imaging of Primary Mouse Embryonic Palatal Mesenchyme Cells to Analyze Collective Movement Attributes

Published on: February 13, 2021

在小鼠胚胎 palatal 骨发育过程中,骨质细胞分化和动态 mRNA 表达

Yongzhen Lai^1,2, Yan Guo², Caiyu Liao²

¹Department of Oral and Craniomaxillofacial Science, Fujian Medical University Union Hospital, No. 28, Xinquan Road, Fuzhou, 350001, Fujian, China.

Scientific reports

|September 13, 2023

概括

这项研究揭示了胚胎口腔骨中的骨质细胞 (OCL) 差异化,确定了关键信号通路,如PI3K-AKT和MAPK参与骨重塑和发育.

科学领域:

发展生物学发展生物学
骨生物学骨生物学
细胞生物学细胞生物学

背景情况:

胚胎口腔骨中的骨质细胞 (OCL) 差异化和功能在很大程度上仍未被描述.
了解这些过程对于理解口腔骨发育和潜在的先天性异常至关重要.

研究的目的:

研究胚胎 palatal 骨中的OCL分化,功能和相关分子通路.
为了确定关键的基因和信号级联调节OCL发育在这个特定的解剖区域.

主要方法:

在 palatal 组织中的各种胚胎时间点 (E16.5-E18.5) 观察耐酸酸酶 (TRAP) 阳性 OCLs.
在关键的OCL分化时期 (E14.5-E16.5) 对口腔进行RNA测序 (RNA-seq) 分析,以比较大和口腔过程组织.
生物信息分析以确定丰富的信号通路和差异表达的基因.

主要成果:

在胚胎口腔骨中发现了TRAP阳性OCL,在TRAP阳性之前开始分化.
在OCL分化过程中,PI3K-AKT和MAPK信号通路被顺序丰富.
特定的基因 (例如,Csf1r,Ctsk,Fos) 被上调,而其他基因 (例如,Pik3r3,Tgfbr1) 被下调,在 palatal process of the palate (PPP) 和 posterior/anterior parts of the palatal process of the maxilla (PPMX) 的 palatal 过程中.
在PPMX与PPP中的Tnfrsff11b的不同调节表明在定时OCL出现方面发挥了作用.

结论:

骨质结晶体参与胚胎 palatal 骨头重塑,骨髓腔形成和血管化.
PI3K-AKT和MAPK通路对于OCL存活和发育口腔中的差异化至关重要.
特定的基因表达模式,包括Tnfrsff11b的差异调节,有助于在不同的口腔区域中控制OCL分化的时间控制.

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