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柏柏林可以缓解葡萄糖皮质类药物引起的高血糖症:一项体外和体外研究
Mrinal Gupta1, Mohammad Rumman2, Babita Singh1
1Department of Biochemistry, King George's Medical University, Lucknow, Uttar Pradesh, India.
Naunyn-Schmiedeberg's archives of pharmacology
|September 13, 2023
概括
柏柏林 (BBR) 通过改善葡萄糖平衡和减少肥胖,有效地对抗小鼠的德甲诱导的糖尿病. 这种天然化合物显示出作为治疗类固醇诱导糖尿病和相关代谢障碍的治疗剂的前景.
科学领域:
- 药理学 药理学是指药理学的学科.
- 代谢疾病 代谢疾病
- 自然产品研究自然产品研究
背景情况:
- 柏柏林 (BBR) 是一种具有已知的药理作用的生物活性化合物.
- 它的抗糖尿病潜力,特别是在甲 (Dex) 诱导的糖尿病模型中,在很大程度上尚未被探索.
- 自然化合物为传统糖尿病治疗提供了更少副作用的替代方案.
研究的目的:
- 为了研究Berberine (BBR) 的抗糖尿病作用,在甲 (Dex) 诱导的糖尿病的小鼠模型中.
- 探索BBR对葡萄糖代谢和肥胖的作用的潜在机制.
主要方法:
- 德克萨米他 (Dex) 用于在30天内诱导小鼠糖尿病.
- 柏柏林 (BBR) 用于口服,剂量各不相同 (100,200,500毫克/公斤).
- 在体外研究中使用HepG2细胞进行葡萄糖释放和糖原合成测定;在体内研究中包括葡萄糖,胰岛素,酸盐测试和Echo MRI用于脂肪质量分析. 基因表达分析阐明了机制.
主要成果:
- 在体外,BBR (高达50μM) 没有影响HepG2细胞活力,但抑制了肝脏葡萄糖释放,改善了葡萄糖耐受性.
- 在体内,BBR治疗显著改善了糖尿病小鼠的葡萄糖平衡,增强了葡萄糖清除,糖解和葡萄糖吸收,同时减少了葡萄糖生成.
- BBR治疗改善了德克斯诱导的总脂肪质量的增加.
结论:
- 柏柏林 (BBR) 在甲 (Dex) 诱导的糖尿病小鼠模型中显示出显著的抗糖尿病作用.
- BBR通过增强葡萄糖清除,抑制肝脏葡萄糖释放和减少肥胖症来改善葡萄糖耐受性.
- 柏柏林 (BBR) 显示出作为葡萄糖皮质醇诱导糖尿病和相关肥胖症的未来治疗剂的潜力.
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