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在大米 (Oryza sativa L.) 中的DUF568含有的基因家族域未知功能的表征和分析

Kai Chen^1,2,3, Yilin Wang^1,2,3, Xiaoyan Nong¹

¹College of Bioscience and Biotechnology, Yangzhou University, Yangzhou, 225009, Jiangsu, China.

|September 13, 2023

概括

米中的植物特异性DUF568基因家族对于根,叶和茎的发育至关重要. 这些基因参与激素信号和非生物应激反应.

科学领域:

植物分子生物学植物分子生物学
基因组学就是基因组学.
生物化学生物化学

背景情况:

未知功能的域 (DUF) 蛋白质是保存的,在真核生物中未表征的蛋白质家族.
一些DUF与植物发育和非生物应激耐受性有关.
DUF568家族是植物特异的,以前没有描述过.

研究的目的:

在大米中描述DUF568家族.
探索DUF568基因在米发育和应激反应中的功能作用.

主要方法:

在大米,阿拉比多普西斯和玉米中对DUF568成员的家族遗传学分析.
对OsDUF568基因的cis元素预测.
使用预测和RT-qPCR进行基因表达分析.
同表达和蛋白质与蛋白质相互作用网络分析.

主要成果:

来自大米和玉米的DUF568成员显示出比Arabidopsis更密切的进化关系.
在OsDUF568基因中的82%以上的上游元素对光和植物激素做出了反应.
OsDUF568基因在苗组织 (叶子,茎,根) 中表达高.
一些OsDUF568基因的表达因植物激素 (ABA,6-BAP) 和非生物应激 (干旱,冷却) 而有所变化.
基因本体学分析揭示了DUF568相关基因的细胞运输,新陈代谢和发育过程中的丰富.

结论:

OsDUF568家族对大米的根,叶和茎的发展至关重要.
这种基因家族可能参与了酸和细胞因素信号传递.
OsDUF568基因可能有助于大米植物组织中的非生物应激抵抗.

关键词:

DUF568 域名域名表达方式表达模式.基因家族基因家族人类遗传学分析大米大米大米大米大米

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