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Mingze Yao¹, Yuanqing Pan², Tinglin Ren²

¹Institutes of Biomedical Sciences, Shanxi Provincial Key Laboratory for Medical Molecular Cell Biology, Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Shanxi University, Taiyuan, 030006, China. yaomz@sxu.edu.cn.

Stem cell research & therapy

|September 13, 2023

概括

盘相互作用蛋白2同源B (Dip2b) 不是小鼠胚胎干细胞多能或增殖的必要条件. 然而,Dip2b淘汰会通过降低参与轴突指导和突触功能的关键基因来影响神经分化.

科学领域:

神经科学是一个神经科学.
发育生物学发展生物学
遗传学是一个遗传学.

背景情况:

盘相互作用蛋白2同类B (Dip2b) 是Dip2家族的一员,由Dip2b基因编码.
Dip2b在神经相关组织中得到广泛的表达,并且在胚胎发生过程中对轴突外生至关重要.

研究的目的:

研究Dip2b在小鼠胚胎干细胞 (mESC) 多能性和神经分化中的作用.

主要方法:

使用CRISPR/Cas9基因编辑建立了一个Dip2b淘汰赛mESC线.
评估了细胞周期,生长速度,多能性和分化潜力,使用流细胞计,qRT-PCR,免疫光和RNA-seq.

主要成果:

在mESC多能性维护,细胞循环和增殖中,Dip2b是不可或缺的.
在体外,Dip2b淘汰并没有影响mESCs在体外分化成三个生殖层的能力.
与轴突指导,通道活性和突触膜相关的基因在Dip2b淘汰细胞的神经分化过程中被显著下调.

结论:

迪普2b在神经分化中起着重要作用,独立于其在多能性中的作用.
Dip2b淘汰赛mESC模型为研究Dip2b在神经发育中的精确机制提供了一个有价值的系统.

关键词:

阿克森指导指导是指导的.这就是CRISPR/Cas9的作用.这就是Dip2bb.基因淘汰赛基因淘汰赛基因淘汰赛神经分化的神经分化.在RNA-seqqq.mESCs是什么意思

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