相关实验视频

Updated: Jul 16, 2025

Dual CRISPR-Interference Strategy for Targeting Synthetic Lethal Interactions Between Non-Coding RNAs in Cancer Cells

Dual CRISPR-Interference Strategy for Targeting Synthetic Lethal Interactions Between Non-Coding RNAs in Cancer Cells

Published on: May 30, 2025

在人类癌症中非编码RNAs/DNMT3B轴:从致病源到临床意义

Chunjie Huang¹, Paniz Azizi², Masoud Vazirzadeh³

¹Institute of Reproductive Medicine, School of Medicine, Nantong University, Nantong, 226001, China.

Journal of translational medicine

|September 13, 2023

概括

研究DNA甲基转移酶3β (DNMT3B) 和非编码RNA (ncRNAs) 之间的相互作用为癌症发展提供了新的见解. 了解这一轴对于推进癌症预防和治疗策略至关重要.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.

背景情况:

癌症的发病包括复杂的遗传和表观遗传变化.
DNA甲基化模式,特别是涉及DNA甲基转移酶3β (DNMT3B),在瘤中经常受到失调.
非编码RNAs (ncRNAs),包括miRNAs,lncRNAs和circRNAs,在基因调节中起着至关重要的作用,并与癌症发病有关.

研究的目的:

为癌症中ncRNAs/DNMT3B轴提供全面的概述.
为了阐明由这一轴驱动的瘤发生的复杂机制.
为了确定癌症干预的潜在治疗点.

主要方法:

对DNMT3B和癌症中的ncRNAs现有文献的综述.
分析ncRNAs和DNMT3B之间的相互调节机制.
综合了关于ncRNAs/DNMT3B轴对癌细胞特征的影响的数据.

主要成果:

DNMT3B的失调是瘤预后和分期的重要指标.
异常的ncRNA表达极大地影响癌细胞的行为,如转移,增殖和耐药性.
ncRNAs和DNMT3B之间的相互作用显示出对瘤细胞命运的协同作用.

结论:

ncRNAs/DNMT3B轴是癌症发病的一个关键参与者.
准这一轴对开发新型癌症疗法充满希望.
对这一轴的进一步研究可以在癌症预防和治疗方面取得重大进展.

关键词:

癌症癌症癌症癌症在DNMT3B中,我们可以使用DNMT3B.没有编码的RNAs.

更多相关视频

Continuous Fluorescence-Based Endonuclease-Coupled DNA Methylation Assay to Screen for DNA Methyltransferase Inhibitors

Continuous Fluorescence-Based Endonuclease-Coupled DNA Methylation Assay to Screen for DNA Methyltransferase Inhibitors

Published on: August 5, 2022

CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis

CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis

Published on: April 25, 2022

相关概念视频

lncRNA - Long Non-coding RNAs

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...

The Nucleolus

The Nucleolus

The nucleolus is the most prominent substructure of the nucleus. When it was first discovered, it was considered to be an isolated organelle that forms fibrils and granules. In 1931, the relationship between the nucleolus and chromosomes was first described by Heitz. He observed that the appearance and size of nucleolus varies depending on the stage of the cell cycle. He also noticed constricted regions on different chromosomes clustered together at definite cell cycle stages. These regions,...

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...

Epigenetic Regulation

Epigenetic Regulation

Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
X-chromosome...

mTOR Signaling and Cancer Progression

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...

Abnormal Proliferation

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...