关于牛短暂发烧病毒的codon使用偏差模式的全面检查
Swati Rani1, M N Mamathashree1, Uma Bharthi I1
1Disease Informatics, Spatial Epidemiology Lab, ICAR - National Institute of Veterinary Epidemiology and Disease Informatics, Bengaluru, India.
Journal of biomolecular structure & dynamics
|September 14, 2023
概括
分析了牛短暂发烧病毒 (BEFV) 的演变,揭示了受自然和突变压力影响的适度的密码使用偏差. 在G和P基因中发现了重组信号,有助于理解病毒病原和疫苗设计.
科学领域:
- 兽医病毒学 兽医病毒学
- 分子进化分子进化
- 基因组学就是基因组学.
背景情况:
- 牛短暂发烧病毒 (BEFV) 严重影响牲畜,造成经济损失.
- 人们对BEFV的进化轨迹和全球传播的了解仍然很少.
- BEFV感染会影响牛和水牛的牛奶和牛肉生产.
研究的目的:
- 阐明BEFV基因 (G,M,N,P) 的进化模式,包括同义代码的使用.
- 为了研究BEFV基因组内的地力学和重组事件.
- 了解基因表达和共同适应,以改善疾病管理和疫苗开发.
主要方法:
- 遗传学分析被用来构建一个病毒进化树.
- 进行了重组分析,以确定潜在的重组信号.
- 编码器使用偏差分析,包括有效编码器数 (eNC) 和中性图,对选定的BEFV基因进行.
主要成果:
- 重组信号仅在BEFV的G和P基因中检测到.
- 在BEFV基因中,Codon使用偏差是由自然选择和突变压力形成的,其中核酸A占主导地位.
- 观察到适度的编码子使用偏差 (eNC值42.9947.10),P基因显示最高偏差,G基因显示最低偏差.
- 中立性和PR-2图表表明强大的选择压力影响了子使用模式.
结论:
- BEFV演变的特点是适度的代使用偏差和显著的选择压力.
- 在G和P基因的重组在BEFV的分子进化中起作用.
- 研究结果为BEFV病变,基因表达提供了关键的见解,并为预防策略和疫苗设计提供了信息.
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