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在癌症细胞系中,染色体外圆形DNA的变异

Carl Rung Dos Santos¹, Lasse Bøllehuus Hansen¹, Monica Rojas-Triana¹

¹Ecology and Evolution, Department of Biology, University of Copenhagen, Denmark.

Computational and structural biotechnology journal

|September 14, 2023

概括

外染色体圆形DNA (eccDNA) 在癌症发育中至关重要. 然而,它们的序列含量在癌症细胞系中是高度可变的,这影响了研究的可重复性.

科学领域:

在瘤学瘤学.
遗传学是一个遗传学.
分子生物学分子生物学

背景情况:

携带瘤基因的外染色体圆形DNA (eccDNA) 与癌症发展和患者生存率低下有关.
癌细胞系和瘤活检是研究eccDNA作用的常见模型,但在体外培养过程中eccDNA损失引发了可再生性问题.

研究的目的:

为了全面分析七种不同的癌症细胞系内的和之间的eccDNA变异性.
评估使用癌细胞系用于研究癌症研究中的eccDNA的可靠性.

主要方法:

分析了七个癌细胞系 (MCA3D,PDV,HaCa4,CarC,MIA-PaCa-2,AsPC-1和PC-3) 的三重复制中的eccDNA含量.
在细胞系复制体中比较独特的eccDNA含量和序列共同性.
在来自不同来源的PC-3细胞系分离物中研究携带MYC的eccDNA (NCI与ATCC).

主要成果:

独特的eccDNA的数量是细胞系特异性的.
在三重复 (0-1%的共同性) 中观察到eccDNA序列含量的显著变化.
在NCI PC-3分离物中发现了大量的MYC携带的eccDNA,但在ATCC分离物中没有.

结论:

癌症细胞系在它们的eccDNA序列含量中表现出很高的变异性.
在研究中使用之前,对癌细胞系进行eccDNA的彻底测试是必不可少的.
对含有eccDNA的特定亚克隆进行丰富对于在癌症中进行准确的eccDNA研究获得纯种群至关重要.

关键词:

在CNV中,CNV是NV.癌症细胞系癌症细胞系双分钟是双分钟的时间.在EcDNADNA中使用.EccDNADNA 这就是我们的DNA.可复制性可复制性非曼德勒派的非曼德勒派

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