完整的线粒基因组的Anaplectoides virens (巴特勒,1878) (Lepidoptera: Noctuidae) 和家族遗传学分析
Zhaohui Pan1, Sicheng Huang1, Weilie Zheng1
1Key Laboratory of Forest Ecology in Tibet Plateau, Ministry of Education (Institute of Plateau Ecology, Tibet Agricultural & Animal Husbandry University), Linzhi, China.
Mitochondrial DNA. Part B, Resources
|September 14, 2023
概括
属Anaplectoides的第一个完整的线粒体基因组是使用基因组扫描进行测序的. 这为Noctuidae家族内的基因关系提供了新的见解.
科学领域:
- 昆虫学 昆虫学是一门学科.
- 基因组学就是基因组学.
- 分子遗传学分子遗传学
背景情况:
- 阿纳普莱克托伊德 (Anaplectoides virens) 是一种多食草食,会影响各种作物.
- 目前对Anaplectoides的分子遗传学研究是有限的.
研究的目的:
- 为了呈现第一个完整的线粒体基因组为Anaplectoides属.
- 分析Anaplectoides在Noctuidae家族中的遗传定位.
主要方法:
- 用基因组脱皮来获得测序数据.
- 生物信息组件被用来重建线粒基因组.
- 用组装的线粒体基因组进行了家族遗传学分析.
主要成果:
- 阿纳普莱克托伊德的完整线粒体基因组是15358个基因组,其中有37个基因 (13个蛋白质编码,2个rRNA,22个tRNA,控制区域).
- 确定了特定的启动 (cox1的ATN,CGA) 和停止编码子 (cox1,cox2,nad4的TAA,T).
- 遗传学分析支持Anaplectoides,Agrotis和Striacosta在Noctuinae中形成一个单体群.
- 在Noctuidae中Noctuinae,Hadninae和Amphipyrinae之间的关系得到了强烈的支持.
结论:
- 测序的Anaplectoides线粒基因组是未来分子遗传学研究的宝贵资源.
- 这项研究阐明了Noctuidae家族内的Anaplectoides的进化关系.
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