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甲杆菌 (Apilactobacillus kunkeei) 释放与RNA相关的膜囊泡和蛋白质纳米粒子

Christian Seeger¹, Karl Dyrhage¹, Kristina Näslund¹

¹Molecular Evolution, Department of Cell and Molecular Biology, Science for Life Laboratory, Biomedical Centre, Uppsala University, 752 36 Uppsala, Sweden.

|September 14, 2023

概括

分析了蜜蜂共生体Apilactobacillus kunkeei的细胞外颗粒. 研究人员在膜囊泡和细胞外颗粒中确定了关键蛋白质和RNA,揭示了它们的功能.

科学领域:

微生物学微生物学
细菌的共生是一种共生.
主体-微生物相互作用

背景情况:

细胞外粒子,包括膜囊泡,在细菌社区功能和宿主相互作用中起着至关重要的作用.
这些颗粒的组成和功能在细菌物种甚至菌株之间存在很大差异.
阿皮拉科巴西勒 (Apilactobacillus kunkeei) 是蜜蜂中发现的一种防御性共生体,强调了它的生态重要性.

研究的目的:

为了确定两种Apilactobacillus kunkeei.ei菌株的膜囊泡和蛋白质颗粒的组成.
为了研究RNA和蛋白质在无细胞颗粒中的分布.
识别和功能预测细胞外蛋白及其相应的基因.

主要方法:

密度梯度超离心法用于将膜囊泡与细胞外颗粒分离.
使用液体染色学-并联质谱法 (LC-MS/MS) 进行了比较蛋白质组学分析.
细胞外RNA被分析并映射到整个细胞转录组.

主要成果:

观察到RNA和蛋白质峰值的明显分离,RNA集中在膜囊泡部分,蛋白质集中在细胞外粒子部分.
总共确定了37种具有I型信号的蛋白质,在两种菌株中都存在29个正义基因.
功能分析预测了细胞外蛋白质中含有甘氨酸酶,甘氨酸转移酶,巨型蛋白质和酶的存在.

结论:

这项研究提供了膜囊泡和来自Apilactobacillus kunkeei.细胞外蛋白的详细组成分析.
这些发现提供了对这种细菌和蜜蜂之间的共生关系背后的分子机制的见解.
了解这些细胞外成分对于破译细菌社区动态和宿主相互作用至关重要.

关键词:

亚比拉科巴西勒斯 (Apilactobacillus kunkeeiei) 是一个有趣的植物.冷电子断层扫描 (Cryo-electron tomography) 是一种使用冷电子断层扫描的技术.电子显微镜的电子显微镜膜囊泡中的膜泡.纳米颗粒是一种纳米粒子.蛋白质组学蛋白质组学翻译学翻译学翻译学翻译学

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