中性粒细胞外细胞陷和中性粒细胞相关介质在人类甲状腺癌中
Luca Modestino1,2,3, Leonardo Cristinziano1,2,3, Remo Poto1,2,3
1Department of Translational Medical Sciences (DiSMeT), University of Naples Federico II, Naples, Italy.
Frontiers in immunology
|September 14, 2023
概括
中性粒细胞外细胞陷 (NETs) 和相关介质显示出作为甲状腺癌 (TC) 严重程度的生物标志物的潜力. 高水平的NET生物标志物,如MPO-DNA复合体和核细胞被观察到在先进的TC,与恶性瘤相关.
科学领域:
- 免疫学 免疫学 免疫学
- 在瘤学瘤学.
- 生物化学 生物化学
背景情况:
- 多态核中性粒细胞 (PMNs) 是炎症和甲状腺癌 (TC) 的关键参与者.
- 在PMN中释放的介质包括骨髓氧化酶 (MPO),素-3 (PTX3),矩阵金属蛋白酶-9 (MMP-9) 和中性粒细胞外细胞陷 (NET).
- NETs和中性粒细胞衍生媒介正在被研究为TC的潜在生物标志物.
研究的目的:
- 评估中性细胞外细胞陷 (NETs) 和相关介质作为甲状腺癌 (TC) 患者的潜在生物标志物.
- 评估不同甲状腺癌亚型和对照组中NET生物标志物和中性粒细胞衍生媒介的血清度.
主要方法:
- 分析了来自患有分化甲状腺癌 (DTC),分化甲状腺癌 (De-DTC),多节腺 (MNG) 和健康对照组 (HCs) 的患者的血清样本.
- NET生物标志物包括dSDNA,核体,素基因素H3 (CitH3) 和MPO-DNA复合体被量化.
- 使用ELISA测量中性粒细胞介质 (MPO,PTX3,MMP-9,CXCL8,GM-CSF) 的情况.
主要成果:
- 与HC患者相比,De-DTC患者的NET生物标志物增加. 在De-DTC和DTC患者中,CitH3水平增加,而不是HC和MNG患者.
- 与HC,MNG和DTC相比,在De-DTC患者中,MPO-DNA复合体和核细胞显著更高.
- 与HC相比,在DTC和De-DTC患者中观察到MPO,PTX3,MMP-9和GM-CSF的水平增加. NET生物标志物与疾病严重程度,年龄和转移状态相关.
结论:
- MPO-DNA复合体,核细胞和CitH3水平可能表明恶性瘤和甲状腺癌的进展.
- 与良性和健康对照人群相比,PMN相关媒介 (MPO,PTX3,GM-CSF) 的血清度升高与甲状腺癌有关.
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