共振光散射相关性光谱及其在生命科学分析化学中的应用
1School of Chemistry and Chemical Engineering, Frontiers Science Centre for Transformative Molecules, State Key Laboratory of Metal Matrix Composites, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, China.
共振光散射相关谱学 (RLSCS) 提供了一种检测单个纳米粒子的新方法,克服了光方法的局限性. 该技术分析光散射,以测量各种应用中的纳米粒子特性和动态.
科学领域:
- 分析化学 分析化学
- 频谱学是一种光谱学.
- 纳米技术 纳米技术
背景情况:
- 光相关谱学 (FCS) 受到光探针光漂白的限制.
- 单粒子检测需要敏感的方法来分析纳米粒子的行为.
- 共振光散射 (RLS) 为相关性光谱学提供了替代信号源.
研究的目的:
- 介绍和详细介绍共振光散射相关谱 (RLSCS) 作为一种新的单粒子检测技术.
- 介绍RLSCS的理论模型和光学配置.
- 证明RLSCS在各种生物和化学测定中的应用.
主要方法:
- 响应光散射 (RLS) 强度波动的自相对应函数分析,来自扩散纳米粒子.
- 基于共聚焦光学,总内部反射 (TIR) 和暗场照明的RLSCS系统的开发.
- 光/散射交叉相关谱学 (FSCCS) 的实施,用于分子-粒子相互作用研究.
主要成果:
- RLSCS成功地测量了纳米粒子度,扩散系数 (转换和旋转),大小和面积比.
- 通过RLSCS,可以检测同质DNA,免疫测试,亡和纳米运动动力学.
- 时间空间分辨散射相关谱法 (SRSCS,DFSCS) 已开发用于在活细胞中成像RLSCS.
结论:
- RLSCS是一种多功能且强大的单纳米粒子分析技术,克服了FCS光漂白的局限性.
- RLSCS及其先进的成像变体为复杂生物系统的定量分析提供了强大的工具.
- 在RLSCS的未来发展承诺扩大纳米调查的能力.
更多相关视频
12:54Implementation of a Coherent Anti-Stokes Raman Scattering CARS System on a Ti:Sapphire and OPO Laser Based Standard Laser Scanning Microscope
Published on: July 17, 2016
09:46Direct Comparison of Hyperspectral Stimulated Raman Scattering and Coherent Anti-Stokes Raman Scattering Microscopy for Chemical Imaging
Published on: April 28, 2022
相关概念视频
Applications of IR Spectroscopy: Overview
Raman Spectroscopy: Overview
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...
2D NMR: Overview of Heteronuclear Correlation Techniques
Photoluminescence: Applications
Raman Spectroscopy Instrumentation: Overview
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
2D NMR: Heteronuclear Single-Quantum Correlation Spectroscopy (HSQC)
