核因子OsNF-YC5通过调节协同激素信号传递来调节大米种子的发芽
Xinkai Jin1, Xingxing Li1, Zizhao Xie1
1Key Laboratory of Biorheological Science and Technology of Ministry of Education, Bioengineering College, Chongqing University, Chongqing 400044, China.
Plant physiology
|September 14, 2023
概括
核因子-Y (NF-Y) 基因OsNF-YC5通过整合ABA和JA信号通路来调节米种子的发芽. 它的淘汰会增强发芽,而过度表达会减少它,影响压力耐受性.
科学领域:
- 植物生物学 植物生物学
- 分子遗传学 分子遗传学
- 农业科学 农业科学
背景情况:
- 种子休眠和发芽对于作物的建立和产量至关重要.
- 核因子-Y (NF-Y) 转录因子是已知的植物生长和应激反应的调节者,但它们在种子发芽中的作用在很大程度上尚未被探索.
研究的目的:
- 调查 NF-Y 基因 OsNF-YC5 在大米 (Oryza sativa L.) 种子发芽中的功能.
- 阐明OsNF-YC5调节发芽的分子机制,特别是它与ABA和JA信号通路的相互作用.
主要方法:
- 在大米中基因淘汰和过度表达.
- 在各种激素治疗 (ABA,MeJA) 和压力条件 (盐度) 下进行种子发芽测试.
- 蛋白质与蛋白质相互作用研究 (SAPK9,OsJAZ9) 和基因表达分析 (OsABA8ox3,OsHAK21).
主要成果:
- OsNF-YC5淘汰增加,而过度表达减少,大米种子发芽.
- OsNF-YC5调节对ABA和MeJA的敏感性,与SAPK9进行稳定,与OsJAZ9进行抑制.
- OsNF-YC5激活了ABA催化作用 (OsABA8ox3) 并通过OsHAK21增强了盐应激耐受性,降低了ROS水平.
结论:
- OsNF-YC5在整合ABA和JA信号通路以控制大米种子发芽方面发挥着至关重要的作用.
- OsNF-YC5是非生物压力下的种子发芽的关键调节者,为改善作物弹性提供了潜在的目标.
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