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Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

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Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
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Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization
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从光学偏光度学中对不连续相位物体的波面重建.

Ricardo Legarda-Saenz, Jorge L Flores, Manuel Servin

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    |September 14, 2023
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    概括

    这项研究提出了一种新的方法,用于在相位测量偏变计中检索波面,克服对象不连续性的挑战. 该方法使用L^p-规范最小化来准确地重建波面,而不需要外部参数.

    科学领域:

    • 光学和光子学 在光学和光子学.
    • 计算成像技术的成像
    • 计量学 计量学 计量学

    背景情况:

    • 阶段测量偏向计 (PMD) 对于表面分析至关重要.
    • 在PMD中,从具有不连续性的物体中获取波面是一个重大挑战.

    研究的目的:

    • 开发一种强大的方法来检索PMD中的波面,特别是对于具有非可区分梯度场的表面.
    • 为了解决现有的波重建技术的局限性.

    主要方法:

    • 使用L^p-规范最小化问题集成梯度场.
    • 用快速的数值方法解决得到的非线性偏微分方程.
    • 无参数的重建方法.

    主要成果:

    • 使用合成和实验数据成功进行数值重建.
    • 证明了处理不连续性和非可差异化的梯度场的能力.
    • 该方法独立于外部参数.

    结论:

    • 提出的L^p-规范最小化方法有效地从具有挑战性的表面重建波浪.
    • 这种方法为相位测量偏向计提供了一个强大的,无参数的解决方案.

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  • 该技术对先进的光学计量学应用具有前景.