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相关概念视频

Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

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Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
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相关实验视频

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Transmission of Multiple Signals through an Optical Fiber Using Wavefront Shaping
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基于双波长全息显微镜图像细分的相位补偿算法.

Wei Liu, Siqin Tao, Fuxia Cheng

    Applied optics
    |September 14, 2023
    PubMed
    概括

    一个新的图像细分算法通过准确补偿相位误差来改进双波长数字全息显微镜. 这使得具有复杂结构的微流体芯片能够高质量的3D成像.

    科学领域:

    • 光学显微镜是一种光学显微镜.
    • 计量学 计量学 计量学
    • 微流体学 微流体学

    背景情况:

    • 双波长数字全息显微镜 (DW-DHM) 由于物体表面粗性和结构复杂性而面临相补偿错误.
    • 传统的2π相补偿方法对于复杂的样品是不够的.

    研究的目的:

    • 为DW-DHM开发一种先进的相位补偿算法,克服传统方法的局限性.
    • 为了实现准确和连续的相位分布,以改善微流体设备的3D成像.

    主要方法:

    • 提出了整合图像分割的相位补偿算法.
    • 最初的2π相位补偿,然后是二元化和消除噪音.
    • 基于细分的二进制图像以获得连续相位的区域特定补偿.

    主要成果:

    • 成功地将算法应用于具有可调等值波长的双波长DHM系统.
    • 实现了微流体芯片通道的高质量3D成像.
    • 对于具有已知的结构的对象,证明了连续实相的有效获取.

    结论:

    • 基于图像分割的相位补偿算法有效地提高了DW-DHM中的3D地形准确性.

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    Shaping the Amplitude and Phase of Laser Beams by Using a Phase-only Spatial Light Modulator
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  • 这种方法为微流体系统的高质量成像提供了一种新的方法.
  • 为复杂的微观结构提供更可靠的3D重建.