在多种疾病中与血管化相关的关键基因的in silico分析和验证
Shicheng Li1, Jiangwen Ruan1, Zicong Yang1
1Department of Cardiology, The People's Hospital of Guangxi Zhuang Autonomous Region, Institute of Cardiovascular Sciences, Guangxi Academy of Medical Sciences, Nanning, China.
Cell biochemistry and function
|September 14, 2023
概括
研究人员确定了参与血管化 (VC) 的关键基因和途径,涉及慢性病和动脉样硬化等各种疾病. 这项研究确定了治疗VC的潜在治疗点,而不会影响正常的骨发育.
科学领域:
- 心血管生物学 心血管生物学
- 分子医学是分子医学.
- 基因组学就是基因组学.
背景情况:
- 血管化 (VC) 构成了重大的治疗挑战,特别是在识别可避免正常骨质变异的标时.
- 了解不同条件下的VC背后的分子机制对于开发有效的治疗方法至关重要.
研究的目的:
- 确定用于血管化 (VC) 治疗的新型分子标.
- 在各种VC模型中选差异表达基因 (DEG),包括内皮骨质转变 (EOT),慢性病 (CKD) 和动脉样硬化 (AS).
主要方法:
- 跨多个VC数据集的差异基因表达分析 (GSE167962,GSE159832).
- 途径分析 (KEGG),蛋白质-蛋白质相互作用网络和枢纽基因识别.
- 在DOCA盐高血压小鼠模型中,使用定量逆转录聚合酶连锁反应和免疫组织化学验证交叉DEGs.
主要成果:
- 在EOT和AS诱导的VC中丰富的途径包括PI3K-Akt,ECM受体相互作用和焦点粘附.
- 在CKD和AS诱导的VC中,丰富的途径涉及ECM受体相互作用,PPAR和胆固醇代谢.
- 在EOT,CKD,AS和高血压模型中观察到C4b,Cebpa,Lyz2和Spp1的升级.
结论:
- 这项研究成功地确定并验证了血管化 (VC) 治疗中几个有前途的分子标.
- 这些发现为开发针对VC的向治疗提供了基础,可以避免对正常骨质生成过程产生不良影响.
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