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响应pH的弹性类似多的设计者凝结物

Robbert J de Haas¹, Ketan A Ganar¹, Siddharth Deshpande¹

¹Department of Physical Chemistry and Soft Matter, Wageningen University and Research, 6708 WE Wageningen, The Netherlands.

ACS applied materials & interfaces

|September 14, 2023

概括

研究人员开发了pH响应的弹性类多 (ELP),形成合成生物分子凝聚物. 这些工程凝聚物能够精确控制细胞反应,为先进的合成细胞设计铺平了道路.

科学领域:

生物化学生化学
合成生物学合成生物学
材料科学材料科学材料科学

背景情况:

生物分子凝结物通过液-液相分离来调节细胞功能.
响应刺激的合成凝聚物对于设计具有时空控制的合成细胞至关重要.
设计这样的系统是模仿生物细分的关键.

研究的目的:

设计和评估响应pH的弹性类聚类 (ELP),用于制造合成凝聚物.
研究基于ELP的冷凝的特性,包括它们对pH值和温度的反应.
为了证明在合成细胞区内使用这些凝结物的可行性.

主要方法:

一个响应pH的弹性类似聚类 (ELP) 家族的合成和表征.
评估由特定的pH值范围 (4-7) 和温度 (20-37°C) 触发的凝结物形成.
在微米大小的囊泡内混合凝结物形成的演示,以及与细胞组件的基因融合.

主要成果:

在尖的pH间隔 (ΔpH < 0.3) 中,ELPs迅速形成凝结物.
在4到7之间的临界pH值下,在一个生理温度范围 (20-37°C) 中观察到凝结.
多种ELP类型成功地在囊泡内形成混合缩物,显示功能集成的潜力.

结论:

响应pH的ELP提供了一个可调节的平台,用于创建合成生物分子凝聚物.
这些ELP可以被设计成合成细胞,用于生化反应的时空控制.
开发的系统为合成生物学中可切换pH的功能区提供了一个有希望的方法.

关键词:

生物分子凝聚剂是生物分子的凝聚物.类似于弹性质的聚类.液态 - 液态相隔离方法响应pH的同化反应合成细胞的合成细胞.

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