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相关概念视频

Elastin is Responsible for Tissue Elasticity01:12

Elastin is Responsible for Tissue Elasticity

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Elastic fiber contains the protein elastin along with lesser amounts of other proteins and glycoproteins. The main property of elastin is that it will return to its original shape after being stretched or compressed. Elastic fibers are prominent in elastic tissues found in skin and the elastic ligaments of the vertebral column.
Ligaments and tendons are made of dense regular connective tissue, but in ligaments not all fibers are parallel. Dense regular elastic tissue contains elastin fibers and...
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Production of Elastin-like Protein Hydrogels for Encapsulation and Immunostaining of Cells in 3D
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响应pH的弹性类似多的设计者凝结物.

Robbert J de Haas1, Ketan A Ganar1, Siddharth Deshpande1

  • 1Department of Physical Chemistry and Soft Matter, Wageningen University and Research, 6708 WE Wageningen, The Netherlands.

ACS applied materials & interfaces
|September 14, 2023
PubMed
概括

研究人员开发了pH响应的弹性类多 (ELP),形成合成生物分子凝聚物. 这些工程凝聚物能够精确控制细胞反应,为先进的合成细胞设计铺平了道路.

科学领域:

  • 生物化学 生化学
  • 合成生物学 合成生物学
  • 材料科学 材料科学 材料科学

背景情况:

  • 生物分子凝结物通过液-液相分离来调节细胞功能.
  • 响应刺激的合成凝聚物对于设计具有时空控制的合成细胞至关重要.
  • 设计这样的系统是模仿生物细分的关键.

研究的目的:

  • 设计和评估响应pH的弹性类聚类 (ELP),用于制造合成凝聚物.
  • 研究基于ELP的冷凝的特性,包括它们对pH值和温度的反应.
  • 为了证明在合成细胞区内使用这些凝结物的可行性.

主要方法:

  • 一个响应pH的弹性类似聚类 (ELP) 家族的合成和表征.
  • 评估由特定的pH值范围 (4-7) 和温度 (20-37°C) 触发的凝结物形成.
  • 在微米大小的囊泡内混合凝结物形成的演示,以及与细胞组件的基因融合.

主要成果:

  • 在尖的pH间隔 (ΔpH < 0.3) 中,ELPs迅速形成凝结物.
  • 在4到7之间的临界pH值下,在一个生理温度范围 (20-37°C) 中观察到凝结.
  • 多种ELP类型成功地在囊泡内形成混合缩物,显示功能集成的潜力.
关键词:
生物分子凝聚剂是生物分子的凝聚物.类似于弹性质的聚类.液态 - 液态相隔离方法响应pH的同化反应合成细胞的合成细胞.

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Design and Construction of Artificial Extracellular Matrix aECM Proteins from Escherichia coli for Skin Tissue Engineering
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结论:

  • 响应pH的ELP提供了一个可调节的平台,用于创建合成生物分子凝聚物.
  • 这些ELP可以被设计成合成细胞,用于生化反应的时空控制.
  • 开发的系统为合成生物学中可切换pH的功能区提供了一个有希望的方法.