泰国的狗呼吸道冠状病毒发生突变,证实了基因重组的潜在假定父体
Panida Poonsin1,2, Vorapun Wiwatvisawakorn3, Jira Chansaenroj4
1Department of Pathology, Faculty of Veterinary Science, Chulalongkorn University , Bangkok, Thailand.
Microbiology spectrum
|September 14, 2023
概括
泰国的狗呼吸道冠状病毒 (CRCoV) 显示出不断演变的基因组特征和明显的突变. 一个泰国CRCoV菌株可能是美国菌株的祖先,突出显示正在进行的病毒进化.
科学领域:
- 兽医病毒学 兽医病毒学
- 分子进化分子进化
- 狗的传染病 狗的传染病
背景情况:
- 狗呼吸道冠状病毒 (CRCoV) 是狗感染性呼吸道疾病综合体的关键组成部分.
- 关于全球CRCoV的基因组特征和进化轨迹的理解有限.
研究的目的:
- 来自泰国的CRCoV菌株进行首次全长基因组表征.
- 分析泰国CRCoV随着时间的推移而发生的进化动态和遗传变化.
主要方法:
- 从21个CRCoV序列 (2013-2015年和2021-2022年) 的尖峰 (S) 基因的家族遗传学分析.
- 完整的基因组特征,包括突变的识别和选择性压力分析.
- 与全球CRCoV菌株进行比较分析.
主要成果:
- 泰国CRCoV菌株表现出明显的基因组特征和家族遗传集群,在2013-2015年和2021-2022年期间观察到的变化.
- 在所有泰国CRCoV菌株中发现了一种导致截断非结构蛋白的保存无意义突变.
- B组菌株在S基因中表现出非同义突变的特征,表明正在进行的进化. 一个泰国菌株被确定为美国CRCoV菌株的潜在祖先.
- 冠状病毒的进化似乎是通过净化选择驱动的,估计在1992年出现,并在2004年引入泰国.
结论:
- 泰国CRCoV菌株具有独特的基因组特征,并且经历了显著的进化变化,包括特定的突变.
- 观察到的突变和进化模式表明,CRCoV在没有遗传重组的情况下进化.
- 这些发现为CRCoV基因组多样性和进化提供了关键的见解,对未来的监测和控制工作至关重要.
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