相关实验视频

Updated: Jul 16, 2025

Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons

Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons

Published on: August 29, 2014

一个简单的,单管重叠的片向的Illumina测序试验

Jason D Limberis¹, Alina Nalyvayko¹, Joel D Ernst¹

¹Division of Experimental Medicine, University of California, San Francisco, San Francisco, California, United States of America.

|September 14, 2023

概括

这项研究介绍了发针Illumina单管测序PCR (hissPCR),一种用于病原体识别的新方法. hissPCR扩展了Illumina测序的阅读长度,在单个反应中提高了感染的诊断效率.

科学领域:

分子生物学分子生物学
基因组学就是基因组学.
传染病诊断传染病诊断传染病诊断

背景情况:

针对性安普利康序列测定对于识别病原体,抗菌素耐药性和微生物菌株至关重要.
来自Illumina测序的短读长通常需要分割有限的临床样本,复杂化分析.
现有方法面临的挑战是样本输入和试剂要求,以进行全面的测序.

研究的目的:

开发一种精简的方法来扩展Illumina测序的阅读长度,使用单个反应.
克服短读测序在病原体和耐药性基因识别中的局限性.
为了保持较低的样本输入和试剂要求,同时增强测序数据.

主要方法:

发Illumina单管测序PCR (hissPCR) 的开发和实施.
在单个PCR管中生成包含Illumina索引和适配器的重叠.
应用hisPCR用于病原体识别,抗性突变检测和菌株类型.

主要成果:

他的PCR能够在单个管中生成重叠的amplicons,有效地延长读数长度.
该方法成功地将Illumina索引和适配器集成到片生成过程中.
保持已建立的试剂和样品输入要求,提供更有效的工作流程.

结论:

他的PCR提供了一个有价值的工具,用于增强有针对性的片序列应用程序.
该方法简化了病原体和耐药性分析的工作流程,这对于临床诊断至关重要.
这种方法提供了一种具有成本效益和高效的解决方案,可以克服序列中的短读限制.

更多相关视频

T and B Cell Receptor Immune Repertoire Analysis using Next-generation Sequencing

T and B Cell Receptor Immune Repertoire Analysis using Next-generation Sequencing

Published on: January 12, 2021

Detection of Rare Genomic Variants from Pooled Sequencing Using SPLINTER

Detection of Rare Genomic Variants from Pooled Sequencing Using SPLINTER

Published on: June 23, 2012

相关概念视频

Next-generation Sequencing

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....

RNA-seq

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...