随机结合的基因组N6-甲基脱氧氨酸延迟了在类动物中细胞转录的启动
Febrimarsa1, Sebastian G Gornik1, Sofia N Barreira2
1Centre for Chromosome Biology, School of Biological and Chemical Sciences, University of Galway, Galway, Republic of Ireland.
The EMBO journal
|September 14, 2023
概括
在早期动物胚胎中随机积累N6-甲基脱氧腺素 (6mA),暂时抑制转录. Alkbh1充当基因组6mA清洁剂,确保及时激活胚胎基因.
科学领域:
- 分子生物学分子生物学
- 发展生物学 发展生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
背景情况:
- N6-甲基脱氧腺素 (6mA) 是一种在所有生命形式中发现的DNA修饰.
- 动物基因组中6mA的功能仍在争论中,与其在细菌和原生体中既定的作用不同.
研究的目的:
- 为了研究6mA在cnidarianHydractinia symbiolongicarpus早期胚胎基因组中的作用和动态.
- 确定Alkbh1在6mA清除中的功能及其对胚胎基因组激活的影响.
主要方法:
- 在Hydractinia symbiolongicarpus的早期胚胎发生过程中观察6mA的积累和清除.
- 基因操纵Alkbh1以评估其在6mA动态和转录中的作用.
- 分析不同胚胎阶段的6mA水平,与胚胎转录启动相关.
主要成果:
- 基因组6mA在早期的Hydractinia胚胎中随机积累,在16细胞阶段达到峰值,并在64细胞阶段清除.
- Knockdown 的 Alkbh1 导致在 64 细胞阶段的 6mA 水平升高,并延迟了胚胎转录.
- 数据表明6mA来源于回收的母性RNA,并起到抑制转录的作用,而不是作为表观遗传标记.
结论:
- 在Hydractinia中,6mA不是表观遗传标记,而是暂时抑制胚胎转录.
- Alkbh1对于清除基因组6mA至关重要,从而能够及时激活胚胎基因组.
- 6mA清除的缺陷可能是与基因表达干扰相关的病理的新奇原因.
相关概念视频
02:23RNA Editing
9.0K
RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...
9.0K
01:37Epigenetic Regulation
3.1K
Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
X-chromosome...
X-chromosome...
3.1K
02:30Genomic Imprinting and Inheritance
34.6K
Diploid organisms inherit genetic material through chromosomes from both parents. Copies of the same gene are known as alleles. In most cases, both alleles are simultaneously expressed and allow various cellular processes to function optimally. If one of the alleles is missing or mutated, the expression of the other allele can compensate; however, this is not true for all genes.
The expression of some genes depends on which parent passed the gene to the offspring, through a phenomenon known as...
The expression of some genes depends on which parent passed the gene to the offspring, through a phenomenon known as...
34.6K
03:17Inheritance of Chromatin Structures
6.3K
Epigenetics is the study of inherited changes in a cell's phenotype without changing the DNA sequences. It provides a form of memory for the differential gene expression pattern to maintain cell lineage, position-effect variegation, dosage compensation, and maintenance of chromatin structures such as telomeres and centromeres. For example, the structure and location of the centromere on chromosomes are epigenetically inherited. Its functionality is not dictated or ensured by the underlying...
6.3K