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相关概念视频

Integrins01:10

Integrins

4.0K
Animal and protozoan cells do not have cell walls to help maintain shape and provide structural stability. Instead, these eukaryotic cells secrete a sticky mass of carbohydrates and proteins into the spaces between adjacent cells. This network of proteins and molecules is called an extracellular matrix or ECM.
Some ECM proteins assemble into a basement membrane to which the remaining components adhere. Proteoglycans typically form the bulk of the ECM while fibrous proteins, like collagen,...
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Protein Translocation Machinery on the ER Membrane01:28

Protein Translocation Machinery on the ER Membrane

4.7K
The translocon complex situated on the ER membrane is the main gateway for the protein secretory pathway. It facilitates the transport of nascent peptides into the ER lumen and their insertion into the ER membrane.
Sec61 protein conducting channel
In eukaryotes, the translocon complex comprises a core heterotrimeric translocator channel called the Sec61 complex. This channel includes three transmembrane proteins, Sec61α, Sec61β, and Sec61γ, and is the largest subunit of the...
4.7K
Adherens Junctions01:24

Adherens Junctions

4.8K
Strong contact points between adjacent cells anchor them to each other, forming tissues. Such anchoring junctions are of two types –  adherens junctions and desmosomes. Adherens junctions are abundant in tissues such as  epithelium and endothelium, forming a continuous zone of adhesion called the adhesion belt. In other tissues, such as  heart muscle, they appear as clusters, linking the cells to produce coordinated heart muscle contraction.
Adherens Junctions are Dynamic
4.8K
Selectins01:25

Selectins

3.4K
Cell adhesion is  an essential aspect of multicellularity. While stable cell interactions usually occur between cells of the same type, transient cell interactions occur between cells of different tissue types, such as between neutrophils and endothelial cells. Selectins are one class of cell adhesion molecules (CAMs) that bind carbohydrate ligands to form transient cell adhesion. They are rod-like proteins with a long extracellular part of variable length ending with the lectin domain,...
3.4K
GPI Anchoring of Proteins in the ER Membrane01:29

GPI Anchoring of Proteins in the ER Membrane

4.1K
GPI-anchoring is a post-translational, reversible protein modification that is ubiquitous in eukaryotes. Such proteins are primarily present on the exoplasmic leaflet of the plasma membrane.
GPI-anchor structure
A sequence of 11 enzymatic reactions results in the synthesis of the complete GPI anchor consisting of a hydrophobic and a hydrophilic portion. The hydrophobic portion comprises phosphatidylinositol, while the hydrophilic part comprises polar groups like phosphoethanolamine,...
4.1K
Activation of Integrins01:15

Activation of Integrins

3.5K
Integrins bind ligands and transmit information from outside the cell to inside or vice-versa through an "outside-in signaling" or "inside-out signaling."
In "outside-in signaling," external factors in the extracellular space bind to exposed ligand binding sites on integrins. This causes the inactive protein to undergo a conformational change to become active. Integrins are often clustered on the cell membrane. Repetitive and regularly spaced ligand binding...
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相关实验视频

Updated: Jul 16, 2025

Engineering Cell-permeable Protein
21:08

Engineering Cell-permeable Protein

Published on: December 28, 2009

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工程蛋白质用于细胞进入.

Xiaoyu Feng1, Ruilong Chang1, Haichao Zhu1

  • 1Jiangsu Key Laboratory of Druggability of Biopharmaceuticals and State Key Laboratory of Natural Medicines, School of Life Science and Technology, China Pharmaceutical University, Nanjing 210009, China.

Molecular pharmaceutics
|September 14, 2023
PubMed
概括
此摘要是机器生成的。

工程化细胞透蛋白克服细胞膜障碍,以提供向的输送. 本综述涵盖了蛋白质类型,内体逃生策略和用于增强治疗应用的设计技术.

关键词:
细胞内体逃脱的方法细胞内蛋白质的输送.蛋白质工程工程 蛋白质工程

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Direct Protein Delivery to Mammalian Cells Using Cell-permeable Cys2-His2 Zinc-finger Domains
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Direct Protein Delivery to Mammalian Cells Using Cell-permeable Cys2-His2 Zinc-finger Domains

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Electroporation of Functional Bacterial Effectors into Mammalian Cells
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Electroporation of Functional Bacterial Effectors into Mammalian Cells

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Last Updated: Jul 16, 2025

Engineering Cell-permeable Protein
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Published on: December 28, 2009

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Direct Protein Delivery to Mammalian Cells Using Cell-permeable Cys2-His2 Zinc-finger Domains
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Electroporation of Functional Bacterial Effectors into Mammalian Cells
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Electroporation of Functional Bacterial Effectors into Mammalian Cells

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科学领域:

  • 生物化学和分子生物学
  • 细胞生物学 细胞生物学
  • 药物输送系统 药物输送系统

背景情况:

  • 蛋白质是所有生物过程的基础.
  • 有效的细胞内蛋白质输送对于治疗应用至关重要.
  • 细胞膜是蛋白质进入的重要障碍.

研究的目的:

  • 对工程细胞透蛋白 (eCPPs) 进行审查,以克服细胞障碍.
  • 讨论加强输送蛋白质内体逃逸的策略.
  • 引入用于设计和检测eCPP的新方法.

主要方法:

  • 关于工程细胞透蛋白质的文献综述.
  • eCPP的分类包括细胞透,超电荷蛋白,受体结合蛋白和细菌毒素.
  • 讨论内体体逃生机制:孔隙形成,质子海绵效应和细胞内贩运通路劫持.

主要成果:

  • 确定了各种类型的工程蛋白质,具有细胞透能力.
  • 详细介绍了各种策略,以促进内体体逃生,以改善细胞内输送.
  • 突出新兴技术用于eCPP的设计和检测.

结论:

  • 工程细胞透蛋白质为向细胞内输送提供了有前途的解决方案.
  • 有效的内体逃生策略对于最大限度地提高蛋白质有效性至关重要.
  • 设计和检测技术的进步将加速基于蛋白质的治疗方法的发展.