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Engineering Cell-permeable Protein

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工程蛋白质用于细胞进入

Xiaoyu Feng¹, Ruilong Chang¹, Haichao Zhu¹

¹Jiangsu Key Laboratory of Druggability of Biopharmaceuticals and State Key Laboratory of Natural Medicines, School of Life Science and Technology, China Pharmaceutical University, Nanjing 210009, China.

Molecular pharmaceutics

|September 14, 2023

概括

工程化细胞透蛋白克服细胞膜障碍,以提供向的输送. 本综述涵盖了蛋白质类型,内体逃生策略和用于增强治疗应用的设计技术.

科学领域:

生物化学和分子生物学
细胞生物学细胞生物学
药物输送系统药物输送系统

背景情况:

蛋白质是所有生物过程的基础.
有效的细胞内蛋白质输送对于治疗应用至关重要.
细胞膜是蛋白质进入的重要障碍.

研究的目的:

对工程细胞透蛋白 (eCPPs) 进行审查,以克服细胞障碍.
讨论加强输送蛋白质内体逃逸的策略.
引入用于设计和检测eCPP的新方法.

主要方法:

关于工程细胞透蛋白质的文献综述.
eCPP的分类包括细胞透,超电荷蛋白,受体结合蛋白和细菌毒素.
讨论内体体逃生机制:孔隙形成,质子海绵效应和细胞内贩运通路劫持.

主要成果:

确定了各种类型的工程蛋白质,具有细胞透能力.
详细介绍了各种策略,以促进内体体逃生,以改善细胞内输送.
突出新兴技术用于eCPP的设计和检测.

结论:

工程细胞透蛋白质为向细胞内输送提供了有前途的解决方案.
有效的内体逃生策略对于最大限度地提高蛋白质有效性至关重要.
设计和检测技术的进步将加速基于蛋白质的治疗方法的发展.

关键词:

细胞内体逃脱的方法细胞内蛋白质的输送.蛋白质工程工程蛋白质工程

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